Ddk Outline
Outline of the Main Points to be included in your Poster Presentation of your article
WHY: Briefly describe the purpose of your article.
-To prove that CDK-S and DDK work together to start meiotic recombination in yeast.
WHAT: What do you hope to learn from reading this article?
-I hope to learn in detail the steps and processes needed to prove how CDK-S and DDK work together to initiate meiotic recombination in yeast. I’m also interested in the steps they took to test all the variables that went along with determining their conclusion.
HOW:
1. Skim and outline:
a. What is the overall objective of this investigation?
-The overall objective is to show that an important target of DDK for DSB formation is Mer2. Another important investigation …show more content…
- To figure out if phosphorylation plays an important role in the functions that underline various meiotic processes that include recombination and segregation. Also figuring out in which ways these processes coordinate themselves.
b. What did the investigator hope to learn from doing this investigation?
-They hoped by identifying and defining the kinases, substrates, and phosphorylation sites involved in recombination and segregation that they could better understand the important factors of meiosis.
2. Define unfamiliar terms:
a. What key terms need to be defined before the investigation can be understood and described?
-Mcm2- protein coding gene
- CDKs- multifunctional enzyme that can modify various substrates that are involved in cell cycle progression.
-Holliday junction resolution- formed between sister chromatids or homologous chromosomes. Takes place during the repair of DNA lesions.
3. Critically read and …show more content…
Figure 5- In this figure we look at the preservation of the DDK-CDK combination site that would be present in Mer2’s genes and compare it to other fungus.
Figure 6- This figure shows us a model that lays out the phosphorylation of Mer2 by CDK-S and DDK.
e. Describe 2-3 results that explain, support, or refute the hypothesis of the paper
1. The presence of DDK is needed so that DSB protein, Mer2 can be phosphorylated. In this section in the results it was shown that DDK and CDK-S function together to help phosphorylate Mer2.
2. In order for a full phosphorylation shift in the mobility we need Ser29 and Ser30 of Mer2 to be present. Now they touch on something that is equally as important for the formation of Mer2, which is the presence of Ser29 and Ser30.
3. There must be a negative charge at both S29 and S30 for meiotic DSB formation. They proved the existence of another aspect important for meiotic DSB formation and that is the negative charge.
f. Describe 2-3 conclusions the authors discuss in the
WHY: Briefly describe the purpose of your article.
-To prove that CDK-S and DDK work together to start meiotic recombination in yeast.
WHAT: What do you hope to learn from reading this article?
-I hope to learn in detail the steps and processes needed to prove how CDK-S and DDK work together to initiate meiotic recombination in yeast. I’m also interested in the steps they took to test all the variables that went along with determining their conclusion.
HOW:
1. Skim and outline:
a. What is the overall objective of this investigation?
-The overall objective is to show that an important target of DDK for DSB formation is Mer2. Another important investigation …show more content…
- To figure out if phosphorylation plays an important role in the functions that underline various meiotic processes that include recombination and segregation. Also figuring out in which ways these processes coordinate themselves.
b. What did the investigator hope to learn from doing this investigation?
-They hoped by identifying and defining the kinases, substrates, and phosphorylation sites involved in recombination and segregation that they could better understand the important factors of meiosis.
2. Define unfamiliar terms:
a. What key terms need to be defined before the investigation can be understood and described?
-Mcm2- protein coding gene
- CDKs- multifunctional enzyme that can modify various substrates that are involved in cell cycle progression.
-Holliday junction resolution- formed between sister chromatids or homologous chromosomes. Takes place during the repair of DNA lesions.
3. Critically read and …show more content…
Figure 5- In this figure we look at the preservation of the DDK-CDK combination site that would be present in Mer2’s genes and compare it to other fungus.
Figure 6- This figure shows us a model that lays out the phosphorylation of Mer2 by CDK-S and DDK.
e. Describe 2-3 results that explain, support, or refute the hypothesis of the paper
1. The presence of DDK is needed so that DSB protein, Mer2 can be phosphorylated. In this section in the results it was shown that DDK and CDK-S function together to help phosphorylate Mer2.
2. In order for a full phosphorylation shift in the mobility we need Ser29 and Ser30 of Mer2 to be present. Now they touch on something that is equally as important for the formation of Mer2, which is the presence of Ser29 and Ser30.
3. There must be a negative charge at both S29 and S30 for meiotic DSB formation. They proved the existence of another aspect important for meiotic DSB formation and that is the negative charge.
f. Describe 2-3 conclusions the authors discuss in the