Decolorization Of Remazol Black-B Lab Report

To scrutinize the color potency depth of the azo dye Remazol Black-B decolorizing activity by an effectual bacterial isolates, liquid medium was used. Based on the relative aptitude of the decolorization of diverse isolates, three most excellent performing bacterial isolates (1, 2 & 3) with more than 90% of decolorizing activity were preferred for more experiments (Data not shown).
3.1 Biodecolorization of Remazol Black-B by selected bacterial isolates
Microbial decolorization of Remazol Black-B by the most promising selected bacterial isolates (1, 2 & 3) was corroborated by performing one more experiment in liquid medium at distinctive time frame (Figure 1). It was surveyed that distinctive bacterial isolates had patchy aptitude to eliminate Remazol Black-B in actively growing cultures. The most competent bacterial isolate to decolorize Remazol black-B colorless was by isolate 1 with 98% color degrading efficiency in 10 h incubation interlude while left over isolates exhibited utmost decolorization in 16 h. Isolate 2 was the second largely
It was apparent (Figure 2) that Remazol Black-B azo dye decolorization piercingly improved up to 100 mg L-1 of substrate concentration and utmost removal was observed at 100 mg L-1 of substrate concentration. Then, there was a slow but sure decline in the azo dye decolorization. Isolate 1was the most proficient azo dye removal strain with more or less complete removal of the color i.e., 100% decolorization at 100 mg L-1 and minimum decolorization was recorded at 50 mg L-1 while after 100 mg L-1 substrate concentration, again 1 showed a decreasing trend. Isolate 2 was the second at the rank with 90% decolorization at 100 mg L-1. But, 3 showed different trend from the other isolates, it indicated enhanced decolorization up to 200 mg L-1