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Describe The Difference Between Alanine And Glutamate

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Describe The Difference Between Alanine And Glutamate
The two spots obtained from tube 1 are glutamate and the product of pyruvate which is alanine. The only spot from tube 2 is glutamate. The 2 spots obtained from tube 3 are alanine and the product of α-ketoglutarate which is glutamate. Moreover, the only spot from tube 4 is alanine. Tube 5 is a control group. Therefore, no reactions are occurred, and no spots on the paper. The reaction for tube 2&4 are different from other tubes is because the enzyme transaminases that stayed in the tubes get denatured by the boiling water bath during the experiment. As a result, no transamination reactions are occurred. Only reactants showed on the paper, but no products. There are no 20 amino acids in the liver extract because ethanol is being added to the
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