Determination of Critical Micelle Concentration
| Determination of the Critical Micelle Concentration | | |
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Introduction: Purpose: The purpose of this experiment is to use the Conductometric method to determine the critical micelle concentration of SDS.
Materials and Equipment: * SDS- Sigma Aldrich, Lot: 38H0080, S/N: L-439, * NaCl- Food grade Great Value * SDS 20% solution- Fisher Biotech, S/N: BP1311-200, Lot: 468059 * Wave generator- Pasco Scientific, S/N PI-9587C * Ammeter- Keithley Auto-ranging Multimeter, Model: 175A, S/N: 0690266 * Oscilloscope- Protek 6506 S/N: 650602332 * Heating mantle/mixer- Cimatec 2 Thermolyne, Model: SP46925, S/N: 1069020639876 * Analytical Balance- Denver Instrument Co., A-250, S/N: 100007, P/N: 201169
Procedure:
Part 1: Pure Water * All materials needed were collected. (SDS 20% solution was only SDS attained at this time) * Two carbon rods were dipped in paraffin and completely covered except for the bottom tips to create electrodes. * These were put in series with a waveform generator and an ammeter. * This was incorrectly done the first time. * A solution of the 20% SDS solution was made and tested in the incorrect set up. * It was concluded that the wave generator needed to be adjusted and new SDS needed to be found. * Proper SDS was found and the set up was corrected. * 50 mL of a 0.04 M aqueous solution was made by mixing 50 mL of purified water and 0.58 g of SDS * 50 mL of purified water was placed in a beaker with a stir bar. * The electrodes were rinsed with water and placed into the beaker well below the surface of the water. * The stirrer was turned on to the lowest speed. * The ammeter was set to the 2-mA range and the waveform generator was set at 1 kHz and the wave generator’s square wave properties were checked with the oscilloscope. * A 0.5 mL calibrated pipet was used to deliver one aliquot of the SDS solution to the beaker every 15 seconds until 40
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Introduction: Purpose: The purpose of this experiment is to use the Conductometric method to determine the critical micelle concentration of SDS.
Materials and Equipment: * SDS- Sigma Aldrich, Lot: 38H0080, S/N: L-439, * NaCl- Food grade Great Value * SDS 20% solution- Fisher Biotech, S/N: BP1311-200, Lot: 468059 * Wave generator- Pasco Scientific, S/N PI-9587C * Ammeter- Keithley Auto-ranging Multimeter, Model: 175A, S/N: 0690266 * Oscilloscope- Protek 6506 S/N: 650602332 * Heating mantle/mixer- Cimatec 2 Thermolyne, Model: SP46925, S/N: 1069020639876 * Analytical Balance- Denver Instrument Co., A-250, S/N: 100007, P/N: 201169
Procedure:
Part 1: Pure Water * All materials needed were collected. (SDS 20% solution was only SDS attained at this time) * Two carbon rods were dipped in paraffin and completely covered except for the bottom tips to create electrodes. * These were put in series with a waveform generator and an ammeter. * This was incorrectly done the first time. * A solution of the 20% SDS solution was made and tested in the incorrect set up. * It was concluded that the wave generator needed to be adjusted and new SDS needed to be found. * Proper SDS was found and the set up was corrected. * 50 mL of a 0.04 M aqueous solution was made by mixing 50 mL of purified water and 0.58 g of SDS * 50 mL of purified water was placed in a beaker with a stir bar. * The electrodes were rinsed with water and placed into the beaker well below the surface of the water. * The stirrer was turned on to the lowest speed. * The ammeter was set to the 2-mA range and the waveform generator was set at 1 kHz and the wave generator’s square wave properties were checked with the oscilloscope. * A 0.5 mL calibrated pipet was used to deliver one aliquot of the SDS solution to the beaker every 15 seconds until 40