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Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method

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Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method
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Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method Prepared for: By: Date:

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Abstract
This study is to examine the effects of different types of disinfectants by disk diffusion method using common agents such as Betadine, Clorox, Crest, Kiss My Face, Listerine, and Lysol. Wound care and general cleaning of home surfaces is a part of daily activity for many of the general population. Knowing which agents are effective against the bacteria that most commonly cause infection, disease, and illness allows educated decisions on which agents to use. We compared the bactericidal effectiveness of 6 common agents widely used by the general public against 3 common bacterial organisms.

Introduction Sterilization kills all organisms in or on an object or substance. Disinfectants and antiseptics do not sterilize since many types of organisms and spores are not killed. Disinfectants are described as antimicrobial agents that are used on inanimate objects such as an instrument or structural surfaces. The term antiseptic is usually applied to antimicrobial agents that are used on living tissue such as skin and throat mucosa.

A disinfectant must be capable of killing pathogens while it is in contact with them, so that they cannot grow again when it is removed. This action is said to be cidal (lethal), and it is described according to the type of organisms it kills as bactericidal, sporicidal, fungicidal, virucidal, etc… If the antimicrobial substance merely inhibits the organisms while it is in contact with them, they may be able to multiply again when it is removed. In this case, the agent is said to have static activity and may be described as bacteriostatic, fungistatic, etc…

Microorganisms of different groups are not uniformly susceptible to chemical disinfection due to the structures of their cell walls. As an example, Mycobacterium are more resistant



Cited: Pearson, 2010. Symbiosis Lab 16 Disk Diffusion Assay to Determine Disinfectant and Antiseptic Effectiveness, pp. 89-91. Pearson Custom Publishing Vicki S. Rabenberg, Christopher D. Ingersoll, Michelle A. Sandrey, and Mary T. Johnson. The Bactericidal And Cytotoxic Effects Of Antimicrobial Wound Cleansers. 2002. Centers for Disease Control and Prevention http://www.cdc.gov/nczved/divisions/dfbmd/diseases/ecoli_o157h7/index.html#spread Violet I. Haraszthy, DDS, MS, PhD; Joseph J. Zambon, DDS, PhD;Prem K. Sreenivasan, PhD. The Antimicrobial Efficacy of Commercial Dentifrices Featured in General Dentistry. 2010 Agarry O., Olaleye M.T, and Bello-Michael, C.O. Comparative antimicrobial activities of aloe vera gel and leaf. African Journal of Biotechnology Vol. 4 (12), pp. 1413-1414, 2005. ZDENKA CVETNI, ANDA VLADIMIR-KNE. Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Department of Microbiology Faculty of Pharmacy and BiochemistryUniversity of Zagreb in conjunction with the Croatia and Department of Pharmacognosy Faculty of Pharmacy and Biochemistry University of Zagreb, Croatia. 2004.

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