Differentiate Between Molds And Yeast
Name of Student: Bal Krishna Maharjan
Course: Food Microbiology 17/FA
Describe how to isolate and differentiate between molds and yeasts?
Answer:
Isolation of molds:
Isolation of molds can be done in two ways:
1. Direct isolation:
Under this different methods can be selected depending upon the nature of molds and need of study. The following are the different direct isolation techniques.
a. Direct transfer: Involves simple transfer of a mold from its source to a pure culture, usually consists of picking a small sample of hyphae or spores which is placed on a fresh plate as a point inoculum for colony development and sporulation.
b. Moist chambers: Isolation of molds is more effective when it is kept in moist chambers for one to several weeks …show more content…
YPG media: used for growth of petite mutants and contains glycerol
5. Minimal media: contains yeast nitrogen base without amino acids, supports growth of wild yeast.
3. Spreading using inoculating loop:
4. Yeast subculture preparation.
5. Isolation temperature and growth rate: Optimum temperature for growth - 30ºC but can grow in wide temperature range, growth rate is directly related to the temperature as temperature is lower their growth is also slower and temperature above 30ºC may cause mutation in petite and sporulation is highly inhibited.
6. Isolating Single Colonies: Done by streaking cultures out on an agar plate to dilute the cells on the surface of the agar, overlapping streaks, separation or distribution of cells to form isolated single colonies.
7. Estimating the Number of Yeast Cells in a Culture:
How to differentiate between molds and yeast culture:
Traditional methods:
Yeast and molds can be identified by general outward appearance. Yeasts are generally small colonies with creamy or white color and smooth texture but larger than bacterial colonies Whereas molds often appear grey, green, brown color with fuzzy edges, sac-like structure called sporangia. Round yeast colonies Microscopic view of
Course: Food Microbiology 17/FA
Describe how to isolate and differentiate between molds and yeasts?
Answer:
Isolation of molds:
Isolation of molds can be done in two ways:
1. Direct isolation:
Under this different methods can be selected depending upon the nature of molds and need of study. The following are the different direct isolation techniques.
a. Direct transfer: Involves simple transfer of a mold from its source to a pure culture, usually consists of picking a small sample of hyphae or spores which is placed on a fresh plate as a point inoculum for colony development and sporulation.
b. Moist chambers: Isolation of molds is more effective when it is kept in moist chambers for one to several weeks …show more content…
YPG media: used for growth of petite mutants and contains glycerol
5. Minimal media: contains yeast nitrogen base without amino acids, supports growth of wild yeast.
3. Spreading using inoculating loop:
4. Yeast subculture preparation.
5. Isolation temperature and growth rate: Optimum temperature for growth - 30ºC but can grow in wide temperature range, growth rate is directly related to the temperature as temperature is lower their growth is also slower and temperature above 30ºC may cause mutation in petite and sporulation is highly inhibited.
6. Isolating Single Colonies: Done by streaking cultures out on an agar plate to dilute the cells on the surface of the agar, overlapping streaks, separation or distribution of cells to form isolated single colonies.
7. Estimating the Number of Yeast Cells in a Culture:
How to differentiate between molds and yeast culture:
Traditional methods:
Yeast and molds can be identified by general outward appearance. Yeasts are generally small colonies with creamy or white color and smooth texture but larger than bacterial colonies Whereas molds often appear grey, green, brown color with fuzzy edges, sac-like structure called sporangia. Round yeast colonies Microscopic view of