2. Development of Microbiology as a Scientific Discipline 1.1 History i) ii) iii) iv) v) vi) Credit – 2 Early observation of microorganisms Spontaneous generation conflict : Contribution of scientists Recognition of the microbial role in diseases. Koch’s postulates Recognition of microbial role in fermentations. Discovery of microbial effects on organic and inorganic matter. Pure culture concept.
vii) Aseptic surgery General characteristics of microorganisms. 2.1 i) ii) iii) iv) General principles ( Bacteria ) Taxonomic rank Classification system Numerical taxonomy Major characteristics of used in taxonomy. Metabolic, Morphological, Etiological. Physiological, Compositions Immunological, proteins,
composition of nucleic acids,
hybridization, nucleic acid sequencing, identification of organisms based on 16srRNA sequencing, 16S rDNA sequencing v) Bergey’s manual of systematic Bacteriology,General characteristics enlisting all parts with major characters & examples. ( Vol I to IV) 2.2 General characteristics and classification of Viruses.(LHT system) 2.3 General characters of Fungi ( including yeasts ) 2.4 General characters of Actinomycetes
2.5 General characters of Algae 2.6 General characteristics of Mycoplasma and Rickettssia. 2.7 General characteristics of Archaebacteria 2.8 Introduction to Gnotobiology/ axenic life/ space microbiology. Credit – 3 : 3.1 Microscopy 3.1 Properties of light i) ii) iii) iv) v) Definitions : Magnification, resolving power, depth of focus, focal length, numerical aperture. Objectives Low , high & oil immersion. Oculars : function, Huygenian, Ramsden, Hyperplane & compensating. Condensers ; Abbe, variable focus cordiod, parabolic & their functions. Iris diaphragm. comparative study of : i) ii) i) ii) iii) iv)