Dna Extraction From Ticks Lab Report

2.4. DNA extraction from ticks and blood samples DNA extraction from ticks was performed by using the QIAamp DNA Mini kit (Qiagen, Germany, GmbH). Ticks washed with 1% SDS, then 180 μl of ATL buffer was added and 20 μl QIAGEN protease. For homogenization of samples, tubes were placed into the adaptor sets, which fixed into the clamps of the Qiagen tissue Lyser. Then they incubated at 56˚C till lysis. After lysis, 200 µl of the lysate was incubated with 10 µl of proteinase K and 200 µl of lysis buffer at 72OC for 10 min. After incubation, 200 µl of 100% ethanol was added to the lysate. Nucleic acid was eluted with 100 µl of elution buffer provided in the kit. DNA extraction from blood was performed using the QIAamp DNA Mini kit (Qiagen,