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Dna Extraction Lab Write Up

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Dna Extraction Lab Write Up
Brandon Schmetterer
3-13-15
Biology labs DNA Extraction Lab
DNA is extracted from humans for genetic testing, for body identification, and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next, the cells must be burst open in order to release DNA. Third, DNA is separated from protein and debris. Lastly, the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis solution to the tube and suck in and transfer liquids .Lysis solution is bursts open cells in order for them to release DNA. The warm water frees the DNA from cells .Concentrated salt solution causes the protein and cellular debris to cumulate together. Micro centrifuge separate protein from DNA .Isopropyl alcohol allows DNA to be visible without any assistance mechanisms .Resuspension buffers are mixed with lysis and isolate DNA .Ethanol precipitates nucleic acids out of solution. Sample tubes are used to hold the samples. …show more content…
The tube is placed into the centrifuge .In the centrifuge, cellular debris sink to the bottom of the tube and are separated from DNA .The tube is extracted from the centrifuge and the micropipettor removes the top liquid that has the DNA into an empty tube .Isopropyl alcohol is added to the tube .The tube is inverted, mixing the isopropyl into DNA solution .DNA is now clumped and visible .The tube is placed into the centrifuge .Lastly, the liquid is removed from the DNA and is dried. DNA extraction is
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