Dna Lab
The investigation on the average mass of DNA with the mass of banana, strawberry and kiwi.
Purpose:
The purpose of this lab is to investigate the comparison between the amount of DNA per gram of fruit that can be extracted from a banana, strawberry and kiwi and to determine which one has more DNA.
Hypothesis:
The banana genome contains 837 MBPs and the strawberry genome contains 206 MBPs and the kiwi genome contains 128 MBPs. This states that there are more base pairs in a banana genome than the strawberry genome and less kiwi genome compare to strawberry genome and banana genome that means that there is more DNA as well.
Therefore, the average mass of DNA will be more in the Banana than the average mass of DNA in a strawberry and kiwi. Also, there will be less average mass of DNA in the kiwi compare to strawberry and banana.
Variables:
Independent variable:
The independent variable for this lab is the number of strawberries, bananas and kiwis that we used to extract the DNA from.
Dependent Variable:
The dependent variable for this lab is the average mass of DNA that we measured for this lab. We did five trials for each fruits and calculated the average mass of DNA from the certain mass of banana/strawberry/kiwi.
Controlled Variable:
The controlled variable for this lab is the number of pieces of fruits we used for the lab and also the amount of warm salt water. We put 25ml of warm salt-water solution into the beaker to be mixed with the crushed fruit. The other controlled variable for this lab is the amount of detergent we put in the mixture of salt water and crushed fruits which is 10ml.
Procedure:
Materials and Apparatus: * * Banana (1 piece) * Strawberry (1 piece) * Kiwi (1 piece) * Electronic Balance * 2 Beakers * 25 ml of warm salt * 10 ml of detergent * Cheese cloth * Ice cold alcohol * Test tube * Pencil * spoon * Wood stir stick * A glass funnel * Calculator *
Purpose:
The purpose of this lab is to investigate the comparison between the amount of DNA per gram of fruit that can be extracted from a banana, strawberry and kiwi and to determine which one has more DNA.
Hypothesis:
The banana genome contains 837 MBPs and the strawberry genome contains 206 MBPs and the kiwi genome contains 128 MBPs. This states that there are more base pairs in a banana genome than the strawberry genome and less kiwi genome compare to strawberry genome and banana genome that means that there is more DNA as well.
Therefore, the average mass of DNA will be more in the Banana than the average mass of DNA in a strawberry and kiwi. Also, there will be less average mass of DNA in the kiwi compare to strawberry and banana.
Variables:
Independent variable:
The independent variable for this lab is the number of strawberries, bananas and kiwis that we used to extract the DNA from.
Dependent Variable:
The dependent variable for this lab is the average mass of DNA that we measured for this lab. We did five trials for each fruits and calculated the average mass of DNA from the certain mass of banana/strawberry/kiwi.
Controlled Variable:
The controlled variable for this lab is the number of pieces of fruits we used for the lab and also the amount of warm salt water. We put 25ml of warm salt-water solution into the beaker to be mixed with the crushed fruit. The other controlled variable for this lab is the amount of detergent we put in the mixture of salt water and crushed fruits which is 10ml.
Procedure:
Materials and Apparatus: * * Banana (1 piece) * Strawberry (1 piece) * Kiwi (1 piece) * Electronic Balance * 2 Beakers * 25 ml of warm salt * 10 ml of detergent * Cheese cloth * Ice cold alcohol * Test tube * Pencil * spoon * Wood stir stick * A glass funnel * Calculator *
Links: chemistry.about.com/science/chemistry/library/weekly/aa061598a.htm * http://www.springerlink.com/content/v544482233771333/