Dolosigranulum Pigrum Lab Report

Dolosigranulum pigrumis considered as an emerging opportunistic pathogen. It was isolated from a patien twith facial herpes zoster accompanied by herpes zoster conjunctivitis and identified by 16S rRNA DNA sequence analysis. Furthermore, literature data were collected together to describe the characteristics of D. pigrum and the infections.
Dolosigranulum pigrum is catalase-negative gram-positive cocci arranged in pairs, tetrads, and clusters and usually colonize the normal floras of the oral cavity, the skin, and the respiratory and alimentary tracts (1). However, there have been very few reports about this bacterium. Here we report D. pigrum associated with a facial herpes zoster accompanied by herpes zoster conjunctivitis in a patient hospitalized
The secretion yielded gram-positive cocci after 2-day incubation at 37°C on 5% sheep-blood agar under anaerobic atmosphere.Biochemical testing gave a positive result of α hemolysis and a negative result for production of catalase. The cocci wereidentified by using the BD Phoneix-100 system (Becton Dickinson, New Jersey, USA) as Alloiococcus otitis, by using VITEK 2-compact system (bioMérieux, Lyons, France)as Micrococcus Kristinae, and by using matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (Bruker, Leipzig, Germany) as “not reliable identification”.Sequencing the 16S rDNA PCR product obtained from the isolate yielded 1406/1419 bases (99%) sequence similarity with the homologous sequence of D. pigrum reference strain (GenBank accession no. X70907.1) by BLAST analysis
pigrum infection cases reported up to now(Table 1) (2-9). The sex of 29 of them is known and 17 were male. The age of 25 of them is known, ranging from 2 months to 92 years. Seven patients were under 3 years and 17 patients were over 60 years, suggesting this D. pigrum more easily infected infants and the elderly people, regardless of gender. D. pigrum were isolated from blood of 15 patientsand eye of 10 patients. The isolates of remaining eight patients were from spinal cord (n=1), sputum (n=1), sinus (n=1), urine (n=1), stomach (n=1), bronchial aspirate (n=1), synovial biopsy (n=1), purulent secretion (n=1). The clinical diagnosis of 20 of them is known. They were diagnosed as eye diseases (n=6), sepsis (n=5), pneumonia (n=3, one of them was complicated by septicemia), sinusitis (n=1), arthritis (n=1), synovitis (n=1), acute cholecystitis accompanied by acute pancreatitis