Dr Zahid Anwar
Pak. J. Bot., 44(1): 169-176, 2012.
OPTIMIZATION OF DILUTE ACID PRETREATMENT USING RESPONSE SURFACE METHODOLOGY FOR BIOETHANOL PRODUCTION FROM CELLULOSIC BIOMASS OF RICE POLISH
ZAHID ANWAR1, MUHAMMAD GULFRAZ1, MUHAMMAD IMRAN1, M. JAVAID ASAD1, AFTAB IQBAL SHAFI1, PERVEZ ANWAR1 AND RAHMATULLAH QURESHI2,*
2 1 Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi Department of Botany, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
Abstract Lignocellulosic biomass is abundant and a renewable resource for the production of biofuel (bioethanol) by using fermentative organisms (Sacchromyces cervesiae & Fusarium oxysporum). Rice polish is a cheaper agro-waste for bioethanol production. The conversion of biomass into maximum yield of glucose, an important step for the bioethanol production, requires optimum dilute acid treatment. Inhibitory compounds reduce the ethanol production; therefore an attempt has been made in the present study to select suitable dilution by using Response surface methodology (RSM) design and to minimize the effects of inhibitory compounds during sulphuric acid (H2SO4) dilution. The treatment of biomass with H2SO4 (1.5%) at 100°C for 30 minutes exhibited optimum results. During enzymatic hydrolysis 16.52 mg/mL glucose was obtained by using 1 mL of enzymatic load at 50oC after 72 hours of hydrolysis. The Glucose thereafter converted to 5.21g/L and 3.69 g/L of ethanol during fermentation process by using Sacchromyces cervesiae and Fusarium oxysporum respectively.
Introduction A variety of lignocellulosic materials like agricultural residues, municipal and industrial wastes are being investigated for bioethanol production (Ying et al., 2009). Cellulose is an abundant biopolymer on the earth and is considered the best renewable energy resource (Rezaei et al., 2008). The major limitations exist for the productions of ethanol from agricultural wastes due to physical and chemical associations
OPTIMIZATION OF DILUTE ACID PRETREATMENT USING RESPONSE SURFACE METHODOLOGY FOR BIOETHANOL PRODUCTION FROM CELLULOSIC BIOMASS OF RICE POLISH
ZAHID ANWAR1, MUHAMMAD GULFRAZ1, MUHAMMAD IMRAN1, M. JAVAID ASAD1, AFTAB IQBAL SHAFI1, PERVEZ ANWAR1 AND RAHMATULLAH QURESHI2,*
2 1 Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi Department of Botany, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
Abstract Lignocellulosic biomass is abundant and a renewable resource for the production of biofuel (bioethanol) by using fermentative organisms (Sacchromyces cervesiae & Fusarium oxysporum). Rice polish is a cheaper agro-waste for bioethanol production. The conversion of biomass into maximum yield of glucose, an important step for the bioethanol production, requires optimum dilute acid treatment. Inhibitory compounds reduce the ethanol production; therefore an attempt has been made in the present study to select suitable dilution by using Response surface methodology (RSM) design and to minimize the effects of inhibitory compounds during sulphuric acid (H2SO4) dilution. The treatment of biomass with H2SO4 (1.5%) at 100°C for 30 minutes exhibited optimum results. During enzymatic hydrolysis 16.52 mg/mL glucose was obtained by using 1 mL of enzymatic load at 50oC after 72 hours of hydrolysis. The Glucose thereafter converted to 5.21g/L and 3.69 g/L of ethanol during fermentation process by using Sacchromyces cervesiae and Fusarium oxysporum respectively.
Introduction A variety of lignocellulosic materials like agricultural residues, municipal and industrial wastes are being investigated for bioethanol production (Ying et al., 2009). Cellulose is an abundant biopolymer on the earth and is considered the best renewable energy resource (Rezaei et al., 2008). The major limitations exist for the productions of ethanol from agricultural wastes due to physical and chemical associations