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E. Coli Lab Report

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E. Coli Lab Report
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In this experiment, we examined the efficiency of transformation of E. coli by introducing plasmids lux and pUC18. A total of six LB plates (3 ampicillin and 3 w/out ampicillin) were used to cultivate bacteria E. coli. The results are as listed: Plates LBlux, LBc, and LBNP exhibited lawn growth. LBNP served as the positive control of the experiment. Plates LB/Ampc and LB/Amplux exhibited colonial growth. The LB/AmpNP plate served as the negative control and exhibited no growth. The calculations for transformation efficiency are provided in Table 1.

Table 1. Transformation efficiency calculations

Total amount of plasmid DNA used
C (pUC18) (0.003ug/μL x 3μL) 0.0009μg lux (0.003ug/μL x 3μL) 0.0009μg
Total volume of cell suspension in
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