Ecoli lab report 
Mallory Charland
February 9, 2015
Mutations of
E. coli Lab Report
Advanced Biology
Deducing Mutations of the Lac Operon of
E. coli
Abstract:
In this lab we determined a possible mutation in unique bacterial strains of
E. coli by observing
the proper responses of wild type
E. coli
Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes, each containing a different substance (lactose, glucose, water and lactose and glucose). After a warm water bath, Sarkosyl detergent, toluene and ONPG were added to the tubes and incubated again. The data was qualified by observing the color change from clear to yellow to indicate the presence of lactase. The results showed that strain A produced no lactase under any circumstance and strain B produced lactase under all circumstances. Introduction, Background and Objectives:
Despite their differences in form and function, all the cells in a multicellular organism have a complete set of genes in each cell’s chromosomes. Because of this, cells have to contain mechanisms that allow them selectively express their genetic information based upon only the tasks at hand. This is very important because many biological phenomena depend on this selective gene expression, such as producing proteins in response to changes in the chemical and physical environments of a cell.
A cluster of functionally related genes, called the lac operon, found within E. coli is responsible for creating enzyme that catabolize lactose. Since lactose is a disaccharide, monosaccharides are produced from this catabolic reaction and used for immediate energy within the cell.
1
Escherichia coli , abbreviated
E. coli
, is a Gram negative, anaerobic, bacterium, that is commonly found in the lower intestine of warm blooded organisms.
“E. coli and related bacteria possess the ability to transfer
DNA via bacterial conjugation
,
transduction or
February 9, 2015
Mutations of
E. coli Lab Report
Advanced Biology
Deducing Mutations of the Lac Operon of
E. coli
Abstract:
In this lab we determined a possible mutation in unique bacterial strains of
E. coli by observing
the proper responses of wild type
E. coli
Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes, each containing a different substance (lactose, glucose, water and lactose and glucose). After a warm water bath, Sarkosyl detergent, toluene and ONPG were added to the tubes and incubated again. The data was qualified by observing the color change from clear to yellow to indicate the presence of lactase. The results showed that strain A produced no lactase under any circumstance and strain B produced lactase under all circumstances. Introduction, Background and Objectives:
Despite their differences in form and function, all the cells in a multicellular organism have a complete set of genes in each cell’s chromosomes. Because of this, cells have to contain mechanisms that allow them selectively express their genetic information based upon only the tasks at hand. This is very important because many biological phenomena depend on this selective gene expression, such as producing proteins in response to changes in the chemical and physical environments of a cell.
A cluster of functionally related genes, called the lac operon, found within E. coli is responsible for creating enzyme that catabolize lactose. Since lactose is a disaccharide, monosaccharides are produced from this catabolic reaction and used for immediate energy within the cell.
1
Escherichia coli , abbreviated
E. coli
, is a Gram negative, anaerobic, bacterium, that is commonly found in the lower intestine of warm blooded organisms.
“E. coli and related bacteria possess the ability to transfer
DNA via bacterial conjugation
,
transduction or