What is the effect of temperature on the permeability of beetroot cell membranes?
Plan:-
Independent Variable- Temperature is what I will be changing in the experiment. I want to find out what effect temperature has on the permeability of a cell membrane.
Controlling other Variables- I will use measures to ensure that everything stays the same in my test to gain the best and most reliable results possible
- Same volume of water that I put the beetroot into after heating.
- The beetroot I use will be the same size each time, I will ensure this using a cork borer.
- The beetroot will be heated for the same time for each one, also it will be left in the water for the same time after.
Equipment:-
Beaker
Thermometer
Heat proof mat
Tripod
Bunsen Burner
Cork Borer
10 Beetroot cylinders
Forceps
10 test tubes
Colorimeter
Measuring cylinder
Also to improve the reliability of the test I will repeat the results.
Method
- Heat 200ml of water in a beaker
- Whilst water is heating, place 10cm3 of water into each of ten test tubes. Label 85-45 and place in a water bath at 20 degree Celsius
- Cut beetroot with a cork borer to length of 5cm. Use a borer with a diameter of 1cm
- Place the beetroot cylinder into a beaker of water at 85 degrees, after one minute use forceps to transfer to test tube of water in water bath at 20 degrees celsius
- As water in beaker cools repeat step 3 using fresh cylinder of beetroot.
- Complete this cycle for 10 cylinders
- Leave each cylinder in its test tube for 30 minutes
- After 30 minutes remove beetroot and shake solution well to get the best quality colour
- Take the solutions and transfer to small beakers and place into the colorimeters, change the setting to transmission and take the r reading. Record the results and plot a graph.
Evaluation
My results show that temperature affects the permeability of beetroot cell membranes and makes it more permeable with a higher temperature. The graph shows how the colour of the beetroot solution is stronger with higher temperatures.
This is due to the
Independent Variable- Temperature is what I will be changing in the experiment. I want to find out what effect temperature has on the permeability of a cell membrane.
Controlling other Variables- I will use measures to ensure that everything stays the same in my test to gain the best and most reliable results possible
- Same volume of water that I put the beetroot into after heating.
- The beetroot I use will be the same size each time, I will ensure this using a cork borer.
- The beetroot will be heated for the same time for each one, also it will be left in the water for the same time after.
Equipment:-
Beaker
Thermometer
Heat proof mat
Tripod
Bunsen Burner
Cork Borer
10 Beetroot cylinders
Forceps
10 test tubes
Colorimeter
Measuring cylinder
Also to improve the reliability of the test I will repeat the results.
Method
- Heat 200ml of water in a beaker
- Whilst water is heating, place 10cm3 of water into each of ten test tubes. Label 85-45 and place in a water bath at 20 degree Celsius
- Cut beetroot with a cork borer to length of 5cm. Use a borer with a diameter of 1cm
- Place the beetroot cylinder into a beaker of water at 85 degrees, after one minute use forceps to transfer to test tube of water in water bath at 20 degrees celsius
- As water in beaker cools repeat step 3 using fresh cylinder of beetroot.
- Complete this cycle for 10 cylinders
- Leave each cylinder in its test tube for 30 minutes
- After 30 minutes remove beetroot and shake solution well to get the best quality colour
- Take the solutions and transfer to small beakers and place into the colorimeters, change the setting to transmission and take the r reading. Record the results and plot a graph.
Evaluation
My results show that temperature affects the permeability of beetroot cell membranes and makes it more permeable with a higher temperature. The graph shows how the colour of the beetroot solution is stronger with higher temperatures.
This is due to the