Preview

Energy Production in Yeast Cells - Glycolysis and Fermentation

Better Essays
Open Document
Open Document
1011 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Energy Production in Yeast Cells - Glycolysis and Fermentation
THE EFFECT OF INHIBITORS AND MANIPULATION IN ENERGY PRODUCTION OF YEAST CELLS FOR GLYCOLYSIS AND FERMENTATION

INTRODUCTION

The aim of this experiment was to study the process of alcoholic fermentation in Saccharomyces cerevisiae (yeast cells) and measure the rate of Co2 production during anaerobic breakdown of the respiratory substrate, sucrose, in the yeast cells. The effects of an inhibitor on respiratory enzyme were also looked at. It is hypothesized that the five different reaction mixtures shown in Appendix 1 with their contents, would each have a CO2 production that is not the same. Mixture 1, which was the control, is expected to produce CO2 at a slower rate because it must be converted to glucose first.

There are two ways in which a cell or cells can gain energy – cellular respiration or fermentation. There are two types of fermentation and that is alcoholic fermentation where pyruvate, produced in glycolysis, is converted into lactate, and also lactic fermentation used by plants and some fungi and they convert pyruvate to ethanol and carbon dioxide. Cellular respiration is a fundamental, catabolic process by which food is broken down in order to produce high energy adenosine triphosphate (ATP) molecules. This procedure is carried out by every cell in both plants and animals and is essential for everyday living. There are three main stages to cellular respiration – glycolysis, the citric acid cycle and the electron transport chain.

Glycolysis means “sugar splitting” and takes place within the cytosol of a cell and can continue in both aerobic and anaerobic conditions. Glycolysis is able to produce energy in the absence of oxygen, however, with the presence of oxygen the process is able to have a far more energy efficient oxidative phosphorylation. Glycolysis involves the breaking down of a 6-carbon sugar, glucose, into two smaller 3-carbon sugar molecules of pyruvate (Campbell et al., 2008). Firstly, an ATP molecule is combined with a glucose

You May Also Find These Documents Helpful

  • Good Essays

    30. Name the three stages of cellular respiration. Glycolysis, Krebs (Citric Acid) Cycle, and Electron Transport Chain What are the two main stages for cellular respiration? Glycolysis and Aerobic Respiration Which stage produces the most energy? Electron Transport…

    • 669 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Sci 230 Essay Example

    • 897 Words
    • 4 Pages

    Glycolysis splits the glucose molecule into two pyruvic acid molecules. The Reactants going into the process are Glucose, NAD+ (and ATP to start the reaction). The products are 2 pyruvic acid molecules NADH and a net gain of 2 ATP (2 used 4 produced).…

    • 897 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Cellular respiration is divided into three different stages. Glycolysis, the first stage of cellular respiration, splits simple carbohydrates such as glucose into two molecules of ATP, two molecules of pyruvic acid, and two electron carried that have high energy that are known as NADH. This part of cellular respiration does not need oxygen, therefore ATP can be created by glycolysis, but it only makes a small amount and this method is not the most efficient method. The next stage in cellular respiration is the Citric Acid Cycle. This stage commences when the two pyruvate acids are converted into acetyl CoA. This pyruvate oxidation will produce 2 NADH and then the acetyl CoA will enter…

    • 1940 Words
    • 8 Pages
    Powerful Essays
  • Good Essays

    Respirationlabbackground

    • 733 Words
    • 2 Pages

    Aerobic cellular respiration is a pivotal process in which organisms carry out in order to sustain life. It is characterized by the release of energy from organic compounds by means of chemical oxidation within the mitochondria of the cell. The reactants are glucose and oxygen, and after a series of complex steps, the products of carbon dioxide, water, and ATP + heat are released. Thus, cellular respiration is an exergonic process, since heat energy is released in order to do cellular work. The overall process can be encapsulated by the following equation: C6H12O6 + CO2 6CO2+ 6H2O+ 586 kilocalories of energy/mole of glucose oxidized. This reaction seems very straightforward, however there are numerous enzyme-mediated reactions that occur within it that are not so perceptible from the simplified equation. Cellular respiration consists of three major stages: The first is Glycolysis; (occurring in the cytosol) in which chemical energy is harvested by oxidizing glucose into two 3 carbon molecules of pyruvate, and thus producing a net of 2 ATP molecules through substrate-level phosphorylation, as well as a net of 2 NADH molecules. Subsequently, the Krebs Cycle commences after 2 pyruvate molecules are converted to 2 Acetyl CoA molecules in the intermembrane space of the mitochondria. During the Krebs Cycle (occurring in the mitochondrial matrix)4 CO2 molecules are released, 1 ATP molecule is formed (for each turn of the cycle), and the reduced forms of 6 NADH and 2 FADH carry the electrons to the next step: the Electron Transport Chain. This occurs in the inner membrane of the mitochondria, and consists of many electron carriers that pass electrons (donated by NADH and FADH2) along through a series of redox reactions. At the end of the chain, oxygen acts as a final electron acceptor and it reduced them to form water. A proton motive force, or H+ gradient,…

    • 733 Words
    • 2 Pages
    Good Essays
  • Better Essays

    Cellular respiration includes the processes of glycolysis, krebs cycle, and the electron transport chain. Glycolysis is used to convert glucose to produce two pyruvate as well as 4 ATP’s and 2 NADH but uses 2 ATP to have a net product of 2 ATP and 2 NADH. The krebs cycle converts pyruvate to Acetyl CoA, which produces 2 ATP,8 NADH, and 2 FADH’s per glucose molecule. Electron transport Chain is the last and most important step of cellular respiration, it makes ATP with the movement of electrons from high energy to low energy that makes a proton gradient which makes ATP, this cannot occur unless oxygen is present. Fermentation is an anaerobic process in which converts sugars into acids, alcohol, or alcohol. This process occurs in yeast and bacteria as well as muscle cells that have no oxygen left. In yeast fermentation produces ethyl alcohol and carbon dioxide from glucose and fructose. Fermentation in bacteria cells the process of fermentation produces ethanol, while in human muscle cells fermentation produces lactic acid in cells that have a short…

    • 1719 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Stage one glycolysis means the “splitting of sugar”. Glycolysis is a six carbon glucose molecule which is then broken in half, forming two three carbon molecules. The initial split requires an energy investment of two ATP molecules per glucose. Then the three carbon molecules donate high energy electrons to NAD+, the electron carrier forming NADH. Glycolysis then makes four ATP molecules when the enzymes transfer phosphate groups from fuel molecules to ADP. Glycolysis produces a net of two molecules of ATP per molecule of glucose. Then the pyruvic acid holds most of the energy of glucose and that energy is then harvested for stage two.…

    • 398 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Unit two Biology

    • 7492 Words
    • 30 Pages

    11 Chapter 2: Cellular respiration and ATP synthesis The commercial uses of anaerobic respiration The alcoholic drinks industry is dependent on anaerobic fermentation by yeast, producing alcohol in beer and wine, or alcohol that is distilled to produce spirits, such as rum. There are so many plant sources that are rich in fermentable carbohyrates that there is a vast range of such drinks produced around the world. For rum, the fermentable carbohydrate is sucrose in sugar cane.…

    • 7492 Words
    • 30 Pages
    Powerful Essays
  • Good Essays

    Cell Work Sheet

    • 850 Words
    • 4 Pages

    • What is the role of glycolysis? Include the reactants and the products. Where does it occur?…

    • 850 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Glycolysis is where glucose is split into two molecules of 3-carbon sugars. This produces 2ATP, 2 pyruvic acid, and 2 high energy. In The Citric Acid Cycle, Acetyl CoA is created. NAD and FAD are reduced carrying the high energy electrons to the next stage. In the Electron Transport, high energy electrons are passed to oxygen where ATP is then produced.…

    • 286 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    A solution of yeast and glucose was prepared with different concentration of enzymes. In All the tubes water, 0.3M glucose and yeast was placed; with the exception of some tubes in which 0.2M MgSo4 and 2.5mL and 1.4ML 0.2 NaF was placed respectively. In another tube was placed both MgSO4 and NaF. The different solutions were prepared and placed in separate tubes for the experiment. A 45oC water bath was setup to be used for the fermentation process.…

    • 1006 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Cellular Respiration Lab

    • 452 Words
    • 2 Pages

    The two types of fermentation that are well known are alcoholic fermentation as well as lactic acid fermentation. Fermentation is vital for many organisms, such as yeast and bacteria, because it allows them to obtain energy required to carry on life processes. Alcoholic fermentation is especially important for human beings, as it is used to produce alcoholic beverages, bread, and many other everyday items that are consumed (Alba-Lois, 2010). On the other hand, lactic acid is a waste product of certain bacteria (Lactobacillales), which is utilized to create many dairy products such as yogurt and cheese. In addition, humans can resort to lactic acid fermentation when oxygen is limited, so it is used as an extra source to obtain oxygen. In our experiment we will be using yeast, a single-celled organism that utilizes sugar as a food source, and it produces energy substances through the breakdown of sugar molecules. Specifically, the type of sugar as a source of food, impacts the speed of fermentation in yeast. In this lab, we will calculate the rate of fermentation in yeast with different solutions of sugar, such as sucrose, fructose, and lactose with glucose being the control. It is important to humans that the yeast uses the best sugar source during fermentation, as it creates important everyday items we consume like bread, alcohol, and…

    • 452 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Cell Energy

    • 520 Words
    • 3 Pages

    During glycolysis, a team of enzymes splits glucose and forms two molecules of pyruvic acid. After investing 2 ATP at the start, glycolysis generates 4 ATP directly. More energy is harvested later from high-energy electrons used to form NADH (Simon, Reece, & Dickey, 2010).…

    • 520 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    In this experiment yeast ferment sugars anaerobically. When yeast ferments the sugars anaerobically, however, CO2 production will cause a change in the weight of the sugar/yeast-solution. This raises a further question: What is the effect of different kinds of sugars on the fermentation process of yeast? The fermentation process was followed at a constant temperature and four different types of sugars were used. By measuring the release of carbon dioxide, we could see what type of sugar had the biggest effect on the fermentation process of yeast, which resulted in Sacharose.…

    • 1136 Words
    • 5 Pages
    Powerful Essays
  • Powerful Essays

    Cellular respiration is an ATP-producing catabolic process in which the electron receiver is an inorganic molecule. It is the release of energy from organic compounds by chemical oxidation in the mitochondria within each cell. Carbohydrates, proteins, and fats can all be metabolized, but cellular respiration usually involves glucose: C6H12O6 + 6O2 → 6CO2 + 6H2O + 686 Kcal of energy/mole of glucose oxidized. Cellular respiration involves glycolysis, the Krebs cycle, and the electron transport chain. Glycolysis is a catabolic pathway that occurs in the cytosol and partially oxidizes glucose into two pyruvate (3-C). The Krebs cycle occurs in the mitochondria and breaks down a pyruvate (Acetyl-CoA) into carbon dioxide. These two cycles both produce a small amount of ATP by substrate-level phosphorylation and NADH by transferring electrons from substrate to NAD+. The Krebs cycle also produces FADH2 by transferring electrons to FAD. The electron transport chain is located at the inner membrane of the mitochondria and accepts energized electrons from enzymes that are collected during glycolysis and the Krebs cycle, and…

    • 1687 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Cell Energy

    • 1057 Words
    • 5 Pages

    In glycolysis, the 6-carbon sugar, glucose, is broken down into two molecules of a 3-carbon molecule called pyruvate. This change is accompanied by a net gain of 2 ATP molecules and 2 NADH molecules. Glycolysis occurs in the cytosol (Simon, Reece, & Dickery, 2010).…

    • 1057 Words
    • 5 Pages
    Good Essays