Enzyme Activity: Laboratory Report
LABORATORY REPORT
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Activity:
Name:
Instructor:
Date:
Enzyme Activity cheryl yelton
November 30, 2014
Predictions
1. Sucrase will have the greatest activity at pH 6
2. Sucrase will have the greatest activity at
40 °C (104 °F)
3. Sucrase activity increases with increasing sucrose concentration.
Materials and Methods
Effect of pH on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. amount of substrate (sucrose) present
3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. undefined 5. Explain why denatured sucrase was used as a control. undefined 6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration. undefined Results
Table 1: Effect of pH on Sucrase Activity
1
2
3
average
ph 3
0.1558
0.1901
0.1653
0.17
ph 4
0.325
0.302
0.311
0.313
Optical Density ph 6
0.954
0.939
0.955
0.949
ph 5
0.644
0.66
0.647
0.65
ph 7
0.775
0.82
0.786
0.794
ph 8
0.409
0.39
0.397
0.399
ph 9
0.194
0.175
0.19
0.186
Effect of pH on Sucrase Activity
1. State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH
(Click on the Save a Copy button on the panel above to save your report)
Activity:
Name:
Instructor:
Date:
Enzyme Activity cheryl yelton
November 30, 2014
Predictions
1. Sucrase will have the greatest activity at pH 6
2. Sucrase will have the greatest activity at
40 °C (104 °F)
3. Sucrase activity increases with increasing sucrose concentration.
Materials and Methods
Effect of pH on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. amount of substrate (sucrose) present
3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. undefined 5. Explain why denatured sucrase was used as a control. undefined 6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration. undefined Results
Table 1: Effect of pH on Sucrase Activity
1
2
3
average
ph 3
0.1558
0.1901
0.1653
0.17
ph 4
0.325
0.302
0.311
0.313
Optical Density ph 6
0.954
0.939
0.955
0.949
ph 5
0.644
0.66
0.647
0.65
ph 7
0.775
0.82
0.786
0.794
ph 8
0.409
0.39
0.397
0.399
ph 9
0.194
0.175
0.19
0.186
Effect of pH on Sucrase Activity
1. State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH