Title: Enzyme activity
Objective: To investigate the effect of temperature on amylase activity
Design principle
Background:
Amylase activity products? (show the equation)
Which factors will affect enzyme activity?
How to study the rate of reaction? (e.g. rate of disappearance of substrates or rate of formation of products)
Independent variable: temperature of reaction mixture or at which the enzymatic reaction occurs. It can be varied by setting water bath at different desired temperature ranging from 0oC to100oC).
Dependent variable: rate of amylase activity. It is measured by the rate of disappearance of starch molecules from the reaction mixture.
Controlled variables: volume and concentration of starch solution; volume and concentration of amylase solution same drop size of iodine solutions; same drop size of reaction mixture taken out for iodine test. Control experiment: reaction mixture without either amylase or starch (this can show that the disappearance of starch is due to the action of amylase on starch). Assumption:
1. The disappearance of starch is only due to the digestion of starch by amylase.
2. The rate of reaction is proportional to the inverse of the time taken for the disappearance of starch molecules from the reaction mixture.
Procedure:
Precautions:
Putting amylase solution and starch solution in the water bath of desired temperature for enough time before mixing them together.
Results:
Title: Relative rate of amylase reaction at different temperatures
Temperature
(0oC)
Time taken for the disappearance of starch/t (min)
Relative rate
1/t (min-1)
1st
2nd
3rd
Average
0
Round off to 2 decimal places
21.5 (Room temperature)
40
e.g. 0.08 or
8x10-2
60
80
100
Plot a graph (not a sketch) on a graph paper to indicate the plotting points. Add a title to your graph and label the axes with units e.g. relative rate of amylase reaction ((min-1) and temperature of reaction mixture) 00C.
Discussion:
Describe the trend indicated by your curve.
Explain the trend.
Sources of error:
Ways of improvement
Conclusion:
Refer to the objective and write down your findings:
At low temperature, optimum temperature; high temperature
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