Enzyme Lab Report

Introduction The human body is kept alive due to an immeasurable amount of chemical reactions that occur in perfect synchronization. Also, these reactions take place at an incredibly fast rate; however, without enzymes, these reactions could possibly take several years to complete. An enzyme is a macromolecule, generally a protein, that lowers the activation energy of a reaction without being changed by the reaction, and this causes the reaction to occur much faster than usual (Campbell et al., 2014). The act of speeding of a chemical reaction is called catalysis, and molecules that perform this are called catalyst. Enzymes act as catalysts, and there a many types of enzymes that perform many different functions. Enzymes act by allowing
The tubes were filled with 3 ml of their corresponding pH phosphate buffer. 10 drops of catechol and 10 drops of potato juice, which contains catecholase, were added to each of the seven tubes. The tubes were covered with Parafilm, stood for 5 minutes, and mixed every minute. After the 5 minutes, data was recorded based on the intensity of color with the “0,+,++,+++”
Each test tube was filled with its corresponding pH 7 phosphate buffer and catechol amounts from Table 2, covered with Parafilm, and inverted to mix. Then, 30 drops of diluted potato juice extract, obtained from a stock potato extract 1:1 bottle, was added to each tube. Then, the tubes were incubated at room temperature for 5 minutes and mixed by inversion every minute. After the 5 minutes, the color intensities of the test tubes were recorded with the “0” to “+++” scale. The differing amounts of catechol allowed for varying substrate concentration to be studied, and the potato juice was diluted to produce a limited amount of