Enzyme Lactase Lab Report
Discussion
The primary purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme, which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated, the alternate hypothesis read: the optimal temperature range for lactase activity is between 23 to 25 ℃. The alternate hypothesis presented for this exercise proved to be supported by the data documented.
These results were to be expected; in actuality, the optimal temperature range hypothesized should have been 37 ℃. This is because enzyme lactase activity takes place within the body, and the average body temperature is at 37 ℃ (Santhosh, 2017). Initially, there were going to be a total of four temperatures being tested: 0℃, 23℃, 37℃, and 60℃. However, due to some conflicts with the preparation of the temperature for experimentation, it was ultimately decided to exclude this specific temperature from the trials. The next, most probable possibility for optimal temperature range from the three remaining temperatures that were to be tested was 23-25 ℃. As the …show more content…
For the third trial of the temperature 60℃, the results yielded odd results. The numbers from the spectrophotometer were almost invariable, which raised concern considering that the previous two trials gave more radical changes between the numbers in comparison. Of course, the potential for human errors is high. There are a number of small mistakes that could have been inadvertently made. In future experiments, it would be advisable to widen the number of temperatures being tested to further specify the optimal temperature for enzyme
The primary purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme, which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated, the alternate hypothesis read: the optimal temperature range for lactase activity is between 23 to 25 ℃. The alternate hypothesis presented for this exercise proved to be supported by the data documented.
These results were to be expected; in actuality, the optimal temperature range hypothesized should have been 37 ℃. This is because enzyme lactase activity takes place within the body, and the average body temperature is at 37 ℃ (Santhosh, 2017). Initially, there were going to be a total of four temperatures being tested: 0℃, 23℃, 37℃, and 60℃. However, due to some conflicts with the preparation of the temperature for experimentation, it was ultimately decided to exclude this specific temperature from the trials. The next, most probable possibility for optimal temperature range from the three remaining temperatures that were to be tested was 23-25 ℃. As the …show more content…
For the third trial of the temperature 60℃, the results yielded odd results. The numbers from the spectrophotometer were almost invariable, which raised concern considering that the previous two trials gave more radical changes between the numbers in comparison. Of course, the potential for human errors is high. There are a number of small mistakes that could have been inadvertently made. In future experiments, it would be advisable to widen the number of temperatures being tested to further specify the optimal temperature for enzyme