Enzyme Reaction Lab

The effect of time on enzyme reaction.

Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds, there would be the least amount of H2O2. The enzyme activity mirrored my predictions. Also, similar results occurred at the 50-second mark. Overall this experiment proved that substrate concentration decreased as time increased and eventually levels off when there is no more substrate to work on.

Introduction:
Catalase is
As the time periods increase, we should see a decline in the amount of KMnO4 needed to change the solution from white to brown. This means that less H2O2 is present.
Catalase is especially abundant in liver cells of animals and other vertebrates, an organ that detoxifies many harmful substances, such as hydrogen peroxide.
Hypothesis- If a beaker with 10 mL’s of catalase and 10 mL’s of H2O2 is left for 60 seconds then it will have less H2O2 left then a beaker, containing the same materials, left for 10 seconds. The beaker that was left for 60 seconds will have less substrate (H2O2) because the reaction had more time to occur. As time increases, substrate concentration
gloves
7. lab coat
8. 3 pipets (for catalase, KMnO4, and sulfuric acid)
9. 10 mL’s distilled water
10. timer

Procedure:
1. Label each beaker with the correct time interval from 10-60 seconds in ten second intervals (10, 20, 30, 40, 50, 60). Label the remaining beaker “control”.
2. With the “control” beaker pour 10 mL’s of H2O2, add 10 mL’s of distilled water (in place of the catalase). Finally add the 10 mL’s of sulfuric acid.
3. Suck up 10 mL’s of potassium permanganate in the pipet. Add potassium permanganate one drop at a time to the beaker. After each drop, stir the mixture. Continue to add KMnO4 to the mixture turns brown and stays brown.
4. Invert the pipet and record the amount of KMnO4 left in the pipet. Subtract the remaining amount from the starting amount (10 mL’s). The answer is the amount of H2O2 left in the beaker. Record this amount.
5. In beaker 2 (10 seconds) add the 10 mL’s of H2O2. Next add 10 mL’s of catalase. At the same time, press start on the timer. After 10 seconds add the sulfuric acid, to stop the reaction. Repeat step 3 and 4.
6. Repeat step 5, adding 10 seconds each beaker.

Data:
Table 1: Results of Enzymatic Reactions

Control
10 sec.
20 sec.
30 sec.
40