Enzyme Temperature Lab Report
Effect of Temperature on Enzymes
~Abstract~
In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C, room temperature which was 22°C, body temperature which is 37°C, and 77°C. The total time of each trial was 2 and a half minute, 1 minute for the H2O2 to acclimatize to the temperature, 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen Peroxide to break down into water and oxygen. Therefore the difference of enzyme rate reaction was determined by putting liver into hydrogen peroxide mixed with detergent and the oxygen. The detergent will capture this oxygen visualizing it to be measured, which will provide an indication of reaction time. As said in the hypothesis, as the temperate increases the rate of enzyme activity will increase, reaching its optimum temperature, however as the temperature becomes too extreme the enzymes will become denatured resulting in a decrease of activity. The results supported the hypothesis to a point showing that there was an optimum …show more content…
This is due to having to wait for temperatures of the baths to reach the same temperature for each test and maintain that temperature for the minute and a half the reaction was being recorded. Lack of concentration, and possibly the impatience of the partner led to the result of the second trial, with temperature 77°C, being a significantly different figure than that of the other two tests for the temperature at 77°C. The average difference between the results of trial two (85 mL) and the results of trial one (36 mL) and three (30 mL) was 52 mL. The temperature of trial two was not 77°C; it was a temperature below that, due to the enzyme rate of reaction being higher due to not as many enzymes being denatured at the temperature it was
~Abstract~
In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C, room temperature which was 22°C, body temperature which is 37°C, and 77°C. The total time of each trial was 2 and a half minute, 1 minute for the H2O2 to acclimatize to the temperature, 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen Peroxide to break down into water and oxygen. Therefore the difference of enzyme rate reaction was determined by putting liver into hydrogen peroxide mixed with detergent and the oxygen. The detergent will capture this oxygen visualizing it to be measured, which will provide an indication of reaction time. As said in the hypothesis, as the temperate increases the rate of enzyme activity will increase, reaching its optimum temperature, however as the temperature becomes too extreme the enzymes will become denatured resulting in a decrease of activity. The results supported the hypothesis to a point showing that there was an optimum …show more content…
This is due to having to wait for temperatures of the baths to reach the same temperature for each test and maintain that temperature for the minute and a half the reaction was being recorded. Lack of concentration, and possibly the impatience of the partner led to the result of the second trial, with temperature 77°C, being a significantly different figure than that of the other two tests for the temperature at 77°C. The average difference between the results of trial two (85 mL) and the results of trial one (36 mL) and three (30 mL) was 52 mL. The temperature of trial two was not 77°C; it was a temperature below that, due to the enzyme rate of reaction being higher due to not as many enzymes being denatured at the temperature it was