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The Extraction of Bioethanol from Pineapple (Ananas comosus) Peelings Through Simultaneous Saccharification and Fermentation Using the Yeast Saccharomyces cerevisiae

AN INVESTIGATORY PROJECT
SUBMITTED AS AN ENTRY TO THE
16TH INTERNATIONAL ENVIRONMENTAL PROJECT OLYMPIAD (16TH INEPO)
16. INEPO ÇEVRE PROJE OLİMPİYADI

FATİH KOLEJİ
(FATIH COLLEGE)
ISTANBUL, TURKEY
1-4 JUNE 2008

Avril Rodiel Bries

Quezon City Science High School (Regional Science High School for NCR)
REPUBLIC OF THE PHILIPPINES- CUMHURIYET FİLİPİNLER
School Year 2007-2008

Mr. Noel Pablo Diaz
Research Adviser

Abstract:

On average, 435,000 metric tons of pineapples are produced annually in the Philippines, which is one of the country’s leading commercial fruit products. However, there are a lot of unused excess parts of the pineapple, notably the peelings, which are considered as waste and contribute to the country’s garbage problem.

There is a global oil crisis, as the demand for petroleum increases each year while our supplies are rapidly being depleted. Bioethanol, a principle biofuel, is a natural alternative to gasoline.

One solution to both dilemmas is to produce bioethanol from pineapple plant peelings. This will be made possible by extracting the glucose content of the peelings and fermenting it through the process of Simultaneous Saccharification and Fermentation (SSF) using the yeast sample. Peel samples, coming from Ananas comosus, will undergo a series of physical and chemical analyses to determine the glucose content, which will be utilized to yield bioethanol. The SSF process will be manipulated in terms of fermentation time and buffer level used to determine the best variables for biofuel production.

Introduction

A. Background of the Study

The concept of producing bioethanol from pineapple (Anae ananas comosus) peelings came from the



Bibliography: A.H. Scragg (1998) Biotechnology for Engineers: Biological Systems in Technological Process, Ellis Horwood Limited, Chapter 12, 235-252 Bernice G Bioenergy International, Project Information BIOENERGY. (1992) Ethanol from Biomass Technology Broder, J.D Goldstein, I.S. and J.M. Easter. (1992) An Improved Process for Converting Cellulose to Ethanol. Technical Association of Pulp and Paper Industry Journal. Grethlein, H.E Hans-Jürgen Rehm and Sanaa Hamdy Omar (1993), Biotechnology Volume 1: Biological Fundamentals. Hartfield, S Hester, R.D, S. Hartfield, and G.E. Farina. (1993) “A process for separating acid-sugar mixtures using ion exclusion chromatography.” Proceedings Tenth International Symposium on Alcohol Fuelds, Colorado Srings, Colorado James E. Bailey and David F. Ollis (1986), Biochemical Engineering Fundamentasl 2nd Edition. McGraw Hill. Naim Kosaric, Andrzej Wieczorek, Gregory P Prasner, L. et al, (1986) High-Yield Organosolv Process for Conversion of Cellulosic Biomass to Ethanolin Energy from Biomass and Wastes (D.L. Klass, ed.), Institute of Gas Technology, Chicago, 1279: 1318.

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