Estimation of insoluble dietary fibre in breakfast cereal
1. The aim
The NDF method measure cellulose, hemicellulose and lignin. It is used as an official method for determining dietary fibre in grains and cereals. The method is also applicable for types of feeds and forages. [1]The method consists in a solution of neutral detergent which melt from solid to liquid pectins and plant cell components, such as proteins and lipids.[2] The solution is applied to solubilize proteins. The addiction of the amylase enzyme, which occurs twice, is made in due to broke starch bonds and aid in the analysis. The main disadvantage of this method is that the soluble fibre cannot be measured [1].
2. Results
To calculate the percentage dietary fibre the following equation must be used:
w1- Weight of dried sample; w2- Weight of Glass wool + crucible; w0- Weight of the sample before analysis.
Below there are the weights for the all brand sample and the % dietary fibre:
All Bran
W0
W1
W2
% fibre
Replicate 1
1.071g
69.192g
68.339g
-
Replicate 2
1.069g
71.145g
70.278g
-
Replicate 3
1.062g
71.371g
70.481g
-
Average
1.069g
71.145g
70.278g
-
Through the results obtained, we have that w1 is higher than w2. Showing that there was analysis error, since it is not logical for the final weight to be lower than the Weight of Glass wool + crucible (g).
For reach a better analysis of results this report will address group 11 results for All Bran.
All Bran
W0
W1
W2
% fibre
Replicate 1
1.012g
69.946g
70.331g
38.04
Replicate 2
1.007g
70.264g
70.619g
35.25
Replicate 3
1.004g
70.236g
70.365g
12.85
Average
1.007g
70.236g
70.365g
35.25
The same calculus was made for the Porridge oats sample and the % fibre results are showed in the table below:
Porridge Oats
W0
W1
W2
% fibre
Replicate 1
1.132g
70.782g
70.971g
16.70
Replicate 2
1.126g
70.258g
70.408g
13.31
Replicate 3
1.004g
70.211g
70.359g
14.74
Average
1.126g
70.258g
70.408g
13.32
The NDF method measure cellulose, hemicellulose and lignin. It is used as an official method for determining dietary fibre in grains and cereals. The method is also applicable for types of feeds and forages. [1]The method consists in a solution of neutral detergent which melt from solid to liquid pectins and plant cell components, such as proteins and lipids.[2] The solution is applied to solubilize proteins. The addiction of the amylase enzyme, which occurs twice, is made in due to broke starch bonds and aid in the analysis. The main disadvantage of this method is that the soluble fibre cannot be measured [1].
2. Results
To calculate the percentage dietary fibre the following equation must be used:
w1- Weight of dried sample; w2- Weight of Glass wool + crucible; w0- Weight of the sample before analysis.
Below there are the weights for the all brand sample and the % dietary fibre:
All Bran
W0
W1
W2
% fibre
Replicate 1
1.071g
69.192g
68.339g
-
Replicate 2
1.069g
71.145g
70.278g
-
Replicate 3
1.062g
71.371g
70.481g
-
Average
1.069g
71.145g
70.278g
-
Through the results obtained, we have that w1 is higher than w2. Showing that there was analysis error, since it is not logical for the final weight to be lower than the Weight of Glass wool + crucible (g).
For reach a better analysis of results this report will address group 11 results for All Bran.
All Bran
W0
W1
W2
% fibre
Replicate 1
1.012g
69.946g
70.331g
38.04
Replicate 2
1.007g
70.264g
70.619g
35.25
Replicate 3
1.004g
70.236g
70.365g
12.85
Average
1.007g
70.236g
70.365g
35.25
The same calculus was made for the Porridge oats sample and the % fibre results are showed in the table below:
Porridge Oats
W0
W1
W2
% fibre
Replicate 1
1.132g
70.782g
70.971g
16.70
Replicate 2
1.126g
70.258g
70.408g
13.31
Replicate 3
1.004g
70.211g
70.359g
14.74
Average
1.126g
70.258g
70.408g
13.32
References: 1.. Analise de fribras alimentares. http://www.slideshare.net/Kzona99/anlise-de-fibra-alimentar-aula-diurno (accessed 04 November 2013). 2.. Neutral Detergent Fiber (NDF) . http://uwlab.soils.wisc.edu/files/procedures/forage_ndf.pdf (accessed 04 November 2013).