When exposed to various mediums, Unknown #7 conveyed various results. Streaking on Mannitol Salt produced a yellow color indicating that the organism was a fermenter. Contrastingly streaking on to…
Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media, including MRS broth and nutrient broth, learn about oxygen and temperature requirements for microbial growth and how to control microbial growth.…
The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and, through a series of metabolic tests, identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45, henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment.…
Cited: Tortora, G., J. Funke, B.R., Case, C.C. (2010) Microbiology: An Introduction. Tenth Edition. San Francisco, Pearson Benjamin Cummings.…
Name and Course Section: Avital Gershtein, Section 701 Title: Aseptic Technique & culturing Microbes - Lab # 4…
Purpose: To learn and employ aseptic technique and basic forms of culture media as well as become familiar with the basic requirements of microbial growth and the methods used to control microbial growth.…
To aseptically transfer bacteria from an unknown to a tube of liquid broth you have to flame the loop or wire before you begin to sterilize it. You then remove the caps from the tubes and flame the mouths of the tubes to prevent air-borne contamination. When all this is done, you have to pick up the inoculum or unknown culture by running the sterile loop or wire down into the tube. Once you got the inoculum you drop it into the sterile medium to inoculate it.…
Floyd, Jeanetta and Kennedy, Emily. Laboratory Exercises for Biology 2117 Introductory Microbiology. Chattahoochee Technical College. 2011.…
The bacteria was removed from a single loopful of broth from the tube and streak it into…
An unknown bacterium experiment, this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al., 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by means of the Embden-Meyerhof pathway, they produce plasmid-encoded proteins and that some of them perform mixed acid fermentation, some use enzyme systems like formate dehydrogenase and pyruvate formate-lyase (PFL) The first thing that had to be done was the streak plating to isolate the bacterium, then the next lab multiple tests were ran to help identify which bacterium was in the sample. One of the most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium.…
The eighth test was to find out if the bacteria in question had flagella. The motility test was performed by aseptically inoculating the unknown bacteria into a tube of TSA broth and allowing it to incubate for 24 hours at 37 degrees Celsius. After the TSA broth culture had incubated, it was used to inoculate a tube of motility medium S to the third. The inoculated motility medium was then incubated at 37 degrees Celsius for another 24 hours and observed for red streaks radiating from the stab…
Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons, such as knowing the causative agent of disease, knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study. This study allows the student to exercise their ability to use and understand sterile techniques, transmission, and prevention of microorganisms.…
1. Living organisms play an important role in the recycling of many elements within an ecosystem. One such way this idea can be proven is through the study of carbon in an ecosystem. Plants, for starters, are a major part of this carbon cycle. During photosynthesis, plants remove carbon dioxide from the atmosphere and release oxygen as a waste product. Another major contributor to this vital cycle is the human being. The average human being goes through respiration each day to help maintain homeostasis. Unlike photosynthesis, carbon is the waste product in respiration, while oxygen is removed from the atmosphere. If carbon isn’t cycling through either photosynthesis or respiration, it reacts with water, in the form of carbon dioxide, to produce bicarbonate. The bicarbonate is a source of energy for aquatic producers such as algae and other aquatic vegetation. On similar terms to the formation of bicarbonate, when aquatic organisms respire, the carbon dioxide released from them in turn reacts with water to form bicarbonate. Human activity has also made a huge impact in this cycle. As humans continue to use fossil fuels daily, the amount of carbon dioxide in the air increases. Lastly, one must remember that the amount of bicarbonate in the water is in equilibrium with the amount of carbon dioxide in the air in the carbon cycle.…
The HIPPA regulations address four broad areas. These are privacy, security identifiers, transaction and code sets. The Privacy Rule sets the standard for controlling protected health information (PHI) in different forms. Among others things, the privacy rules state that a patient has the right to access information, request for corrections to be made errors for specific entries, and receive information on how his advice has been used, including those who have accessed it (Armstrong, Kline-Rogers, Jani, Goldman, Fang, Mukherjee, Nallamothu & Eagle 2005). The patients may also request for confidential information, call for limits on access of information, request for confidential information on sensitive matters, complain to the Privacy Officer of an entity if needed and investigate the complaint with the US Department of Health and Human Services Office of Civil Rights if he/she is dissatisfied. The administration has specific rules and regulations under which information may be disclosed.…
On a crisp late October afternoon in Paris Bernard Arnault, Europe's richest man, is talking about his upcoming trip to one of the fastest-growing outposts of his LVMH empire: Mongolia, of all places. "I like to see the reaction of the people in the shops," says the 61-year-old mogul in lightly accented but supple English. Dapper and slim, he wears a navy suit and matching tie made by his flagship fashion label, Christian Dior ( CHDRF.PK - news - people ), and handmade black loafers by Berluti, one of the lesser known but most exclusive of the 60-plus brands in his stable. "I also like to see the competition," he adds. "I am quite competitive. I want to stay ahead and increase our advance."…