Experiment Synthesis Essay
Materials and Methods In the experiment, a frog was obtained and dissected in a way to expose its heart and then tested using Lab Tutor software, a force transducer, and the PowerLab hardware (ADInstruments 2014).
Frog dissection: Frogs that had been killed by being double-pithed were dissected as described in Lab Tutor. The frog was pinned down with the ventral side up. A longitudinal and a lateral incision were made along the frog’s abdomen. The skin was peeled back and the tissue and sternum were cut away so that the thoracic cavity was exposed and the pericardium was carefully cut away and the heart exposed. The frog was occasionally bathed in Frog Ringer’s solution that was an electrolyte solution that contained 6.5 g/L NaCl, 0.41 g/L KCl, 0.12 g/L CaCl2, and 0.20 g/L of NaHCO3.
Force transducer setup: The force transducer was set up on a ring stand with the force transducer mounted on the micropositioner. The force transducer was then connected to PowerLab, zeroed out, and also had its …show more content…
The room temperature (21°C) Frog Ringer’s solution was used to bathe the frog first and the data was recorded for 30 seconds using Lab Tutor. The process was repeated with the warm (37°C) and then the cold (4°C) Frog Ringer’s solutions as well. The frog was bathed in the room temperature solution again before continuing.
Starling’s Law of the Heart: To examine the effects of increasing tension on the heart muscle, the tension of the force transducer was increased by 0.5 mm increments every 10 seconds while recording with Lab Tutor. This was done by slowly turning the micropositioner knob. The process was repeated for 4 more lengths, taking care not stretch the heart too much. When done recording, the micropositioner was returned to its original position to return tension to normal.
The effect of various drug
Frog dissection: Frogs that had been killed by being double-pithed were dissected as described in Lab Tutor. The frog was pinned down with the ventral side up. A longitudinal and a lateral incision were made along the frog’s abdomen. The skin was peeled back and the tissue and sternum were cut away so that the thoracic cavity was exposed and the pericardium was carefully cut away and the heart exposed. The frog was occasionally bathed in Frog Ringer’s solution that was an electrolyte solution that contained 6.5 g/L NaCl, 0.41 g/L KCl, 0.12 g/L CaCl2, and 0.20 g/L of NaHCO3.
Force transducer setup: The force transducer was set up on a ring stand with the force transducer mounted on the micropositioner. The force transducer was then connected to PowerLab, zeroed out, and also had its …show more content…
The room temperature (21°C) Frog Ringer’s solution was used to bathe the frog first and the data was recorded for 30 seconds using Lab Tutor. The process was repeated with the warm (37°C) and then the cold (4°C) Frog Ringer’s solutions as well. The frog was bathed in the room temperature solution again before continuing.
Starling’s Law of the Heart: To examine the effects of increasing tension on the heart muscle, the tension of the force transducer was increased by 0.5 mm increments every 10 seconds while recording with Lab Tutor. This was done by slowly turning the micropositioner knob. The process was repeated for 4 more lengths, taking care not stretch the heart too much. When done recording, the micropositioner was returned to its original position to return tension to normal.
The effect of various drug