Fe2 +/H2o-Mediated Synthesis Lab Report
Summary of Presentation:
Fe2+/H2O- mediated synthesis of Melanins: Cases of Homogentisic acid, serotonin, and norepinephrine
The powerpoint presentation that I attended was titled, “Fe2+/H2O- mediated synthesis of Melanins: Cases of Homogentisic acid, serotonin, and norepinephrine,” the research covered melanin which is a dark pigmentation that can cause other complication and pathological conditions such as, vitiligo and ochronosis. It is believed that melanin protects from ultraviolet light rays, and immobilization of toxic ions. There are several types of melanin; eumelanin and pheomelanin (found mostly in animals as L-DOPA), Neuromelanin (found in brain as dopamine, norepinephrine, and serotonin), Allo Melanin (found in plants, as catechol, and pyrogallol), Pyomelanin (found in fungi or bacteria as homogentisic acid)
Melanin is important to this experiment because, the structure of melanin is not fully known. With more knowledge about this chemical, and the way it function, we would be able to understand what causes it to overproduce and the pathological, and genetic conditions associated with it …show more content…
The small scale experiments were performed three times, where: each compound was mixed with a fixed amount of Fe2+ and various amount of H2O2 and H2O. Solutions were left to react for 3 days, and analyzed by size exclusion chromatography and UV-Vis absorbances were taken. In the large scale experiments, approximately 100 mg of each compound was mixed with fixed amounts of Fe2+ and H20 and varying h2O2 amounts. They sat until the compound reacted away and were analyzed via size exclusion chromatography. They were then purified by dialysis in water for 3 days, changing water several times throughout. Following, they were then frozen and dried using freeze-dry system. Solids retrieved and analyzed using FT-IR
Fe2+/H2O- mediated synthesis of Melanins: Cases of Homogentisic acid, serotonin, and norepinephrine
The powerpoint presentation that I attended was titled, “Fe2+/H2O- mediated synthesis of Melanins: Cases of Homogentisic acid, serotonin, and norepinephrine,” the research covered melanin which is a dark pigmentation that can cause other complication and pathological conditions such as, vitiligo and ochronosis. It is believed that melanin protects from ultraviolet light rays, and immobilization of toxic ions. There are several types of melanin; eumelanin and pheomelanin (found mostly in animals as L-DOPA), Neuromelanin (found in brain as dopamine, norepinephrine, and serotonin), Allo Melanin (found in plants, as catechol, and pyrogallol), Pyomelanin (found in fungi or bacteria as homogentisic acid)
Melanin is important to this experiment because, the structure of melanin is not fully known. With more knowledge about this chemical, and the way it function, we would be able to understand what causes it to overproduce and the pathological, and genetic conditions associated with it …show more content…
The small scale experiments were performed three times, where: each compound was mixed with a fixed amount of Fe2+ and various amount of H2O2 and H2O. Solutions were left to react for 3 days, and analyzed by size exclusion chromatography and UV-Vis absorbances were taken. In the large scale experiments, approximately 100 mg of each compound was mixed with fixed amounts of Fe2+ and H20 and varying h2O2 amounts. They sat until the compound reacted away and were analyzed via size exclusion chromatography. They were then purified by dialysis in water for 3 days, changing water several times throughout. Following, they were then frozen and dried using freeze-dry system. Solids retrieved and analyzed using FT-IR