Florfenicol Lab Report
Determination of Florfenicol in Catfish by UV-mediated High Performance Liquid Chromatography (HPLC)
1. Introduction
1.1 Description of the Analyte
Florfenicol (C12H14Cl2FNO4S) is a derivative of thiamphenicol. Its structure is identical to that of chloramphenicol – a synthetically developed antibiotic for veterinary use, such as treating a wide range of bacterial infections (Hayes p. 7). It is commercially available as 50 percent of Aquaflor® premix, which has been approved for the control of bacteria diseases in aquaculture. Florfenicol has proved to be effective in a broad spectrum of fish species, such as trout, tilapia, and catfish (Wrzesinski, et al. p. 515).
Today, Aquaflor is still used to regulate Edwardsiella ictaluri, which has been identified as …show more content…
Pour 200 mL of C2H3N into a 1 L vacuum flask. After mixing and degasing the solutions, store the mixture for 21 days at room temperature and pressure (r.t.p.).
2.2.3 Solvent Extraction C2H3N-water
Pour 500 mL of water and 500 mL of C2H3N into a 1 L graduated cylinder. Carefully mix the solution and store for 30 days at r.t.p. (Hayes p. 8).
2.2.4 Preparing Florfenicol Strengthening Solutions
2 - 20 mg/mL of reference standard florfenicol solutions should be mixed with ethanol and stored for 14 days at standard conditions of temperature and pressure (Hayes p. 7).
2.2.5 Preparing Standard Stock Solutions of Florfenicol
A stock solution of 1.0 mg/mL florfenicol should be used to develop calibration standards (at least 4) for the samples to be examined (Hayes p. 8). The standard solutions of florfenicol should be tightly stoppered for 14 days at r.t.p. Table 1 illustrates the typical serial dilutions.
Table 1: Sample Dilution Scheme (Courtesy: Hayes p.8)
Nominal Dosage (gkg-1d-1) Weight of the Sample (g) Extraction Solvent (mL) Aliquot Extract Transferred to the
1. Introduction
1.1 Description of the Analyte
Florfenicol (C12H14Cl2FNO4S) is a derivative of thiamphenicol. Its structure is identical to that of chloramphenicol – a synthetically developed antibiotic for veterinary use, such as treating a wide range of bacterial infections (Hayes p. 7). It is commercially available as 50 percent of Aquaflor® premix, which has been approved for the control of bacteria diseases in aquaculture. Florfenicol has proved to be effective in a broad spectrum of fish species, such as trout, tilapia, and catfish (Wrzesinski, et al. p. 515).
Today, Aquaflor is still used to regulate Edwardsiella ictaluri, which has been identified as …show more content…
Pour 200 mL of C2H3N into a 1 L vacuum flask. After mixing and degasing the solutions, store the mixture for 21 days at room temperature and pressure (r.t.p.).
2.2.3 Solvent Extraction C2H3N-water
Pour 500 mL of water and 500 mL of C2H3N into a 1 L graduated cylinder. Carefully mix the solution and store for 30 days at r.t.p. (Hayes p. 8).
2.2.4 Preparing Florfenicol Strengthening Solutions
2 - 20 mg/mL of reference standard florfenicol solutions should be mixed with ethanol and stored for 14 days at standard conditions of temperature and pressure (Hayes p. 7).
2.2.5 Preparing Standard Stock Solutions of Florfenicol
A stock solution of 1.0 mg/mL florfenicol should be used to develop calibration standards (at least 4) for the samples to be examined (Hayes p. 8). The standard solutions of florfenicol should be tightly stoppered for 14 days at r.t.p. Table 1 illustrates the typical serial dilutions.
Table 1: Sample Dilution Scheme (Courtesy: Hayes p.8)
Nominal Dosage (gkg-1d-1) Weight of the Sample (g) Extraction Solvent (mL) Aliquot Extract Transferred to the