Free Fatty Acid Test
FREE FATTY ACID TEST
AIM: To determine seed FFA
MATERIALS REQUIRED Chemcals required * Oxalic acid pellets * Sodium hydroxide pellets * Rectified spirit * Phenolphthalein * Distilled water Apparatus required * 250ml standard flask(oxalic acid) * 1l std flask(sodium hydroxide solution) * 50ml beaker for solution preparation * 250ml beaker for solution preparation * Spatula * 50ml burette with standard for titration * 100ml conical flask * Weighing scale * Hot plate
SOLUTION PREPARATION i)preparation of oxalic acid * take 1.6 gm of oxalic acid pellets in 50 ml beaker and pour distilled water in it * wait to dissolve and then makeup it to 250 ml standard flask ii)preparation of sodium hydroxide solution * take 4gm sodium hydroxide pellets in 250 ml beaker and pour distilled water in it * wait to dissolve then makeup it to 1l standard flask. iii)Titration for normality of NaOH * take 20ml of NaOH solution in 100ml conical flask add 1 drop of phenolphthalein * the colour changes to pink colour * take oxalic acid in 50ml burette and titrate against NaOH solution .The end point is the appearance of pale permanent pink colour.
Formula for normality Normality of NaOH=(burette reading*strength of oxalic acid)/20ml of NaOH
Strength of oxalic acid=amount of OXALIC ACID(gm)* amount of NaOH /mol.wt of oxalic acid
PROCEDURE
a)FOR SEEDS * Take approximately 50-60gm of seed sample to mixi and crushed for 1 or 2 min. * The crushed seeds taken in 100 or 250ml Iodine value(IV) flask * Add 60 to 90ml of Hexane In IV flask and shake it well and close by using stopper * Wait for 5min * Take 50 or 100ml beaker and make the filter paper in round shape and place it beaker * Now pour the Hexane mixed with seed sample in the filter paper * Collect filtered Hexane mixed oil in the beaker and place it in hot plate. * The Hexane evaporates
AIM: To determine seed FFA
MATERIALS REQUIRED Chemcals required * Oxalic acid pellets * Sodium hydroxide pellets * Rectified spirit * Phenolphthalein * Distilled water Apparatus required * 250ml standard flask(oxalic acid) * 1l std flask(sodium hydroxide solution) * 50ml beaker for solution preparation * 250ml beaker for solution preparation * Spatula * 50ml burette with standard for titration * 100ml conical flask * Weighing scale * Hot plate
SOLUTION PREPARATION i)preparation of oxalic acid * take 1.6 gm of oxalic acid pellets in 50 ml beaker and pour distilled water in it * wait to dissolve and then makeup it to 250 ml standard flask ii)preparation of sodium hydroxide solution * take 4gm sodium hydroxide pellets in 250 ml beaker and pour distilled water in it * wait to dissolve then makeup it to 1l standard flask. iii)Titration for normality of NaOH * take 20ml of NaOH solution in 100ml conical flask add 1 drop of phenolphthalein * the colour changes to pink colour * take oxalic acid in 50ml burette and titrate against NaOH solution .The end point is the appearance of pale permanent pink colour.
Formula for normality Normality of NaOH=(burette reading*strength of oxalic acid)/20ml of NaOH
Strength of oxalic acid=amount of OXALIC ACID(gm)* amount of NaOH /mol.wt of oxalic acid
PROCEDURE
a)FOR SEEDS * Take approximately 50-60gm of seed sample to mixi and crushed for 1 or 2 min. * The crushed seeds taken in 100 or 250ml Iodine value(IV) flask * Add 60 to 90ml of Hexane In IV flask and shake it well and close by using stopper * Wait for 5min * Take 50 or 100ml beaker and make the filter paper in round shape and place it beaker * Now pour the Hexane mixed with seed sample in the filter paper * Collect filtered Hexane mixed oil in the beaker and place it in hot plate. * The Hexane evaporates