From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay
Sensors 2009, 9, 6084-6100; doi:10.3390/s90806084
OPEN ACCESS
sensors
ISSN 1424-8220 www.mdpi.com/journal/sensors Article
From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay
Saied Assadollahi 1,2, Christiane Reininger 1,3, Roland Palkovits 1,3, Peter Pointl 1 and Thomas Schalkhammer 1,2,*
1
2
3
Attophotonics Biosciences GmbH, Viktor Kaplan Strasse 2, A-2700 Wiener Neustadt, Austria; E-Mails: palkovits@attophotonics.com (R.P.); Reininger@attophotonics.com(C.R.); pointl@attophotonics.com (P.P.) Max F. Perutz Laboratories, Department of Biochemistry and Molecular Cell Biology, University of Vienna, A-1030 Vienna, Austria; E-Mail: saied.assadollahi@univie.ac.at (S.A.) Departments of Micro and Nanosystems, University of Applied Sciences, A-2700 Wiener Neustadt, Austria; E-Mail: roland.palkovits@fhwn.ac.at (R.P.)
* Author to whom correspondence should be addressed; E-Mail: mail@attophotonics.com; Tel.: +43-2622-23495; Fax: +43-2622-23604 Received: 15 July 2009; in revised form: 24 July 2009 / Accepted: 29 July 2009 / Published: 31 July 2009
Abstract: Improving the performance of traditional diagnostic lateral flow assays combined with new manufacturing technologies is a primary goal in the research and development plans of diagnostic companies. Taking into consideration the components of lateral flow diagnostic test kits; innovation can include modification of labels, materials and device design. In recent years, Resonance-Enhanced Absorption (REA) of metal nano-particles has shown excellent applicability in bio-sensing for the detection of a variety of bio-molecular binding interactions. In a novel approach, we have now integrated REA-assays in a diagnostic microfluidic setup thus resolving the bottleneck of long incubation times inherent in previously existing REA-assays and simultaneously integrated automated fabrication techniques for diagnostics manufacture. Due to the roller-coating based technology and chemical resistance,
OPEN ACCESS
sensors
ISSN 1424-8220 www.mdpi.com/journal/sensors Article
From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay
Saied Assadollahi 1,2, Christiane Reininger 1,3, Roland Palkovits 1,3, Peter Pointl 1 and Thomas Schalkhammer 1,2,*
1
2
3
Attophotonics Biosciences GmbH, Viktor Kaplan Strasse 2, A-2700 Wiener Neustadt, Austria; E-Mails: palkovits@attophotonics.com (R.P.); Reininger@attophotonics.com(C.R.); pointl@attophotonics.com (P.P.) Max F. Perutz Laboratories, Department of Biochemistry and Molecular Cell Biology, University of Vienna, A-1030 Vienna, Austria; E-Mail: saied.assadollahi@univie.ac.at (S.A.) Departments of Micro and Nanosystems, University of Applied Sciences, A-2700 Wiener Neustadt, Austria; E-Mail: roland.palkovits@fhwn.ac.at (R.P.)
* Author to whom correspondence should be addressed; E-Mail: mail@attophotonics.com; Tel.: +43-2622-23495; Fax: +43-2622-23604 Received: 15 July 2009; in revised form: 24 July 2009 / Accepted: 29 July 2009 / Published: 31 July 2009
Abstract: Improving the performance of traditional diagnostic lateral flow assays combined with new manufacturing technologies is a primary goal in the research and development plans of diagnostic companies. Taking into consideration the components of lateral flow diagnostic test kits; innovation can include modification of labels, materials and device design. In recent years, Resonance-Enhanced Absorption (REA) of metal nano-particles has shown excellent applicability in bio-sensing for the detection of a variety of bio-molecular binding interactions. In a novel approach, we have now integrated REA-assays in a diagnostic microfluidic setup thus resolving the bottleneck of long incubation times inherent in previously existing REA-assays and simultaneously integrated automated fabrication techniques for diagnostics manufacture. Due to the roller-coating based technology and chemical resistance,