GE Hw 2
Mujtaba Zafar 10/15/14
Michael Hadjiargyrou Bio 440
Answers only:
Alignment statistics for match #1
Score
Expect
Identities
Gaps
Strand
385 bits(208)
2e-103
229/238(96%)
5/238(2%)
Plus/Plus
Query 1 AGTTATCATGGCCTCCCAAACCACTCACAAAGAAGAAAAAGAGTTTCCAGGAACTAGAAG 60 ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 2774 AGTTATCATGGCCTCCCAAACCACTCACAAAGAAG-AAAAGAGTTTCCAGGAACTAGAAG 2832
Query 61 ATGATTTAGATACCATGGAATTAGAGGATATTGATACAACAGACATCAATCTGGATGAAG 120 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2833 ATGATTTAGATACCATGGAATTAGAGGATATTGATACAACAGACATCAATCTGGATGAAG 2892
Query 121 ATATTCTTGATGACTAAAGAAAGCCCATTTACTCCAGTAAGAGATTATGATGGCACATAC 180 ||||||| ||||||||||| | ||||||||||||||||||||||||||||||||||||
Sbjct 2893 ATATTCTGGATGACTAAAGTTTG-CCATTTACTCCAGTAAGAGATTATGATGGCACATAC 2951
Query 181 AAATCAGTCACCACCCTGACCAGAAACTGTGGCAATCCTTGCTCTGGCAGCACGTAAA 238 ||||||||||||||||||||||| |||||||||||||||||||||| || ||||||||
Sbjct 2952 AAATCAGTCACCACCCTGACCAG-AACTGTGGCAATCCTTGCTCTG-CA-CACGTAAA 3006
Function: This genes main function is to act as a receptor for the activated protein kinase C epsilon and can also be found playing a role in vesicle-mediated transport.
2A) We would use the genomic library because the promoting sequence wouldn’t be found in the cDNA library.
B) For this part I would use the DNA library because it will have the full length DNA sequence. The cDNA would be missing tRNA and rRNA because it is made from the mRNA.
C) I would use the cDNA for this part because we are looking for the expressed DNA so we don’t need all the introns, therefore cDNA would be more ideal.
D) Genomic library since we would be looking at the whole DNA sequence.
E) For this part I will consider using cDNA since bacteria doesn’t carry out post transcriptional modifications so it will have the whole
Michael Hadjiargyrou Bio 440
Answers only:
Alignment statistics for match #1
Score
Expect
Identities
Gaps
Strand
385 bits(208)
2e-103
229/238(96%)
5/238(2%)
Plus/Plus
Query 1 AGTTATCATGGCCTCCCAAACCACTCACAAAGAAGAAAAAGAGTTTCCAGGAACTAGAAG 60 ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 2774 AGTTATCATGGCCTCCCAAACCACTCACAAAGAAG-AAAAGAGTTTCCAGGAACTAGAAG 2832
Query 61 ATGATTTAGATACCATGGAATTAGAGGATATTGATACAACAGACATCAATCTGGATGAAG 120 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2833 ATGATTTAGATACCATGGAATTAGAGGATATTGATACAACAGACATCAATCTGGATGAAG 2892
Query 121 ATATTCTTGATGACTAAAGAAAGCCCATTTACTCCAGTAAGAGATTATGATGGCACATAC 180 ||||||| ||||||||||| | ||||||||||||||||||||||||||||||||||||
Sbjct 2893 ATATTCTGGATGACTAAAGTTTG-CCATTTACTCCAGTAAGAGATTATGATGGCACATAC 2951
Query 181 AAATCAGTCACCACCCTGACCAGAAACTGTGGCAATCCTTGCTCTGGCAGCACGTAAA 238 ||||||||||||||||||||||| |||||||||||||||||||||| || ||||||||
Sbjct 2952 AAATCAGTCACCACCCTGACCAG-AACTGTGGCAATCCTTGCTCTG-CA-CACGTAAA 3006
Function: This genes main function is to act as a receptor for the activated protein kinase C epsilon and can also be found playing a role in vesicle-mediated transport.
2A) We would use the genomic library because the promoting sequence wouldn’t be found in the cDNA library.
B) For this part I would use the DNA library because it will have the full length DNA sequence. The cDNA would be missing tRNA and rRNA because it is made from the mRNA.
C) I would use the cDNA for this part because we are looking for the expressed DNA so we don’t need all the introns, therefore cDNA would be more ideal.
D) Genomic library since we would be looking at the whole DNA sequence.
E) For this part I will consider using cDNA since bacteria doesn’t carry out post transcriptional modifications so it will have the whole