Genetic Research Paper
1 Introduction
The purpose of this research is to compare DNA methylation patterns in two strains of the marine alga, Emiliania Hhuxleyi (Ehux) using Bisulfite sequence. The two strains Ehux-1516 and Ehux-217 share the same genome, but Ehux-1516 is non-calcifying and Ehux-217 is calcifying. Methylation may play a role in the different phenotypes and gene expressions of the two strains. The aim of this research is to analyze the differentially methylation regions (DMR) between the two strains and identify potential links to the expressions of genes related to calcification.
Marine algae are the oldest members of the plant kingdom. Unlike plants, they do not have real roots, stems, leaves, and flowers. They are situated based on food chain …show more content…
We implemented a program for identifying the count of differentially methylated regions on upstream, downstream, intergenic region and anywhere on the gene boundary. We also implemented another program for analyzing overall distribution of differentially methylated cytosines between Ehux-1516 and Ehux-217. A python script was then run to find gene ids of these differentially methylated regions (DMR). Finally, we each CSV files for upstream, downstream, intergenic and genetic regions. These CSV files contains the gene ids of DMRs of Ehux-1516 and Ehux-217 with average methylation level on each gene. IT also includes the starting and ending position of DMR.
The gene ids from the above file with its go terms assigned to Gene Ontology (GO). GO used to describe gene functions. It classifies genes into three functions: cellular component, biological process, and molecular function. After GO, we created a python program for finding the correlation between genes with DMR in gene region, upstream or downstream to those differentially expressed. According to this result, we created a pie chart for analyzing the number of genes included in each
The purpose of this research is to compare DNA methylation patterns in two strains of the marine alga, Emiliania Hhuxleyi (Ehux) using Bisulfite sequence. The two strains Ehux-1516 and Ehux-217 share the same genome, but Ehux-1516 is non-calcifying and Ehux-217 is calcifying. Methylation may play a role in the different phenotypes and gene expressions of the two strains. The aim of this research is to analyze the differentially methylation regions (DMR) between the two strains and identify potential links to the expressions of genes related to calcification.
Marine algae are the oldest members of the plant kingdom. Unlike plants, they do not have real roots, stems, leaves, and flowers. They are situated based on food chain …show more content…
We implemented a program for identifying the count of differentially methylated regions on upstream, downstream, intergenic region and anywhere on the gene boundary. We also implemented another program for analyzing overall distribution of differentially methylated cytosines between Ehux-1516 and Ehux-217. A python script was then run to find gene ids of these differentially methylated regions (DMR). Finally, we each CSV files for upstream, downstream, intergenic and genetic regions. These CSV files contains the gene ids of DMRs of Ehux-1516 and Ehux-217 with average methylation level on each gene. IT also includes the starting and ending position of DMR.
The gene ids from the above file with its go terms assigned to Gene Ontology (GO). GO used to describe gene functions. It classifies genes into three functions: cellular component, biological process, and molecular function. After GO, we created a python program for finding the correlation between genes with DMR in gene region, upstream or downstream to those differentially expressed. According to this result, we created a pie chart for analyzing the number of genes included in each