Process
Recombinant DNA technology begins with the identification and isolation of a gene which expresses a desirable trait, with the aid of restriction enzymes. Then a recipient plant or animal is selected, and the gene is inserted and incorporated into its genome through a vector such as agrobacterium, through a gene gun shooting an elemental particle covered in plasmid DNA, electro oration, or a virus. Once part of the recipient, the newly inserted gene becomes part of the genome of the recipient and is regulated in the same way as its other genes.
For example, we can isolate a gene responsible for conferring drought tolerance, introduce that gene into a plant, and make it drought tolerant. Crops can also be transformed using non-plant genes such as the use of Bt genes, in cotton and many other crops. Bt, or Bacillus thuringiensis, is a naturally occurring bacterium that produces crystal proteins that are lethal only to insect larvae. Bt crystal protein genes have been transferred into cotton, soya, corn, brinjal, enabling the plants to produce its own pesticides against insects such as the American bollworm, European corn