Germinating Yeast Lab
Purpose: The purpose of this lab was to gain a complete understanding around the rate of cellular respiration within multicellular organisms, also to research and understand how to use a CO2.
Background: Living systems require free energy and matter to maintain order, to reproduce, and grow. Energy deficiencies cause disruptions at the population and ecosystem levels as well. 1 mol of H2O produces 1 mol of CO2 through cellular respiration. Autotrophic organisms capture free energy from the environment through the process of photosynthesis and chemosynthesis.
Hypothesis: The germinating beans will discharge more CO2 than the peas that are not germinating.
Materials:
1250 mL respiration chamber
Vernier CO2 gas sensor
Vernier computer interface thermometer
25 non-germinating kidney beans
25, 24 hour germinating kidney beans
25, two hour warm germinating kidney beans
25, two hour cold germinating kidney beans
Paper towels
Safety Hazards: There are no need for goggles or gloves within this lab, but be aware of the choking hazard that the beans can cause.
Procedure:
Gather all materials
Prepare a 100 mL beaker of beans/peas with warm water (warm germinating beans/peas), then prepare a 2nd 100 mL beaker with cold water instead of warm water (cold germinating beans/peas) …show more content…
Configure your CO2 sensor, make sure that it is on its low setting
Plug the CO2 sensor into the vernier data collecting device, configure the device to collect data every 5 minutes
Place 25 non-germinating peas/beans in the bottle, then stick the sensor through the opening
Begin collecting the data through the data collector
After your data has collected reset the collector
Remove the warm germinating beans, dry them off with a paper towel, then place 25 of the beans with in the bottle
Repeat steps 6-7
Repeat steps 3-7 but this time using the cold germinating beans instead of the warm germinating beans
Leave the cold/warm, peas/beans within the water for 24 hours.
Repeat steps 3-7 with the beans that germinated for 48 hours
Data:
Kidney Beans
CO2 ppm (parts per minute)
Time (min)
Control (Dry)
Cold Water
Warm Water
24 Hr.
48 Hr.
0
2170
2183
2168
2166
1667
1
2114
2217
2291
2227
1916
2
2094
2240
2291
2320
2143
3
2094
2236
2326
2404
2363
4
2090
2254
2380
2466
2520
5
2094
2266
2437
2540
2855
Total Change (0-5 min)
-76 ppm
+83 ppm
+269 ppm
+374 ppm
+1,188 ppm
Alaskan Peas
CO2 ppm (parts per minute)
Time
Control (Dry)
Cold Water
Warm Water
24 Hr.
0
2200
1991
1850
2299
1
2060
2022
2021
2759
2
2051
2012
2095
2735
3
2024
2030
2195
2865
4
2024
2063
2302
3138
5
2024
2113
2387
3355
Total Change (0-5 min)
-176 ppm
+122 ppm
+537 ppm
+1,056 ppm
Analysis: While observing the data, the change in CO2 ppm for the control (dry) peas/beans are both moving at a constant rate.
Due to the control, non germinating peas/beans are releasing less CO2 ppm than the 24 hour and 48 hour germinating peas/beans. This can be seen when the control from 0-5 minutes was at -76 ppm, while the 24 hour beans were at +374 ppm. The alaskan peas also saw this same type of pattern from 0-5 minutes. The control for the peas was at -176 ppm and the germinating peas were at +1,056 ppm. Since this lab was conducted with precision, and the sensors were not broken, or tampered with before, during or after the experiment, this data is accurate which leads to precise
calculations.
Conclusion: The results of this lab dp support the hypothesis which is “The germinating beans, and peas will discharge more CO2 than the beans and peas controls.” Since the germinating beans and peas produced more CO2 than the beans/peas that were dry and had not germinated in any water. The reason behind the control not releasing any CO2 because the process called germination occurs when a seed has been soaked in water and has begun releasing CO2 due to the addition of H2O. Seeds that are not soaked within the water are in a dormant state, which leads to the beans/peas releasing less CO2 because no H2O is involved with the process.
Bibliography: "Cellular Respiration." Web. 29 Nov. 2014. . “AP Biology.” Book. 29 Nov .2014..
Background: Living systems require free energy and matter to maintain order, to reproduce, and grow. Energy deficiencies cause disruptions at the population and ecosystem levels as well. 1 mol of H2O produces 1 mol of CO2 through cellular respiration. Autotrophic organisms capture free energy from the environment through the process of photosynthesis and chemosynthesis.
Hypothesis: The germinating beans will discharge more CO2 than the peas that are not germinating.
Materials:
1250 mL respiration chamber
Vernier CO2 gas sensor
Vernier computer interface thermometer
25 non-germinating kidney beans
25, 24 hour germinating kidney beans
25, two hour warm germinating kidney beans
25, two hour cold germinating kidney beans
Paper towels
Safety Hazards: There are no need for goggles or gloves within this lab, but be aware of the choking hazard that the beans can cause.
Procedure:
Gather all materials
Prepare a 100 mL beaker of beans/peas with warm water (warm germinating beans/peas), then prepare a 2nd 100 mL beaker with cold water instead of warm water (cold germinating beans/peas) …show more content…
Configure your CO2 sensor, make sure that it is on its low setting
Plug the CO2 sensor into the vernier data collecting device, configure the device to collect data every 5 minutes
Place 25 non-germinating peas/beans in the bottle, then stick the sensor through the opening
Begin collecting the data through the data collector
After your data has collected reset the collector
Remove the warm germinating beans, dry them off with a paper towel, then place 25 of the beans with in the bottle
Repeat steps 6-7
Repeat steps 3-7 but this time using the cold germinating beans instead of the warm germinating beans
Leave the cold/warm, peas/beans within the water for 24 hours.
Repeat steps 3-7 with the beans that germinated for 48 hours
Data:
Kidney Beans
CO2 ppm (parts per minute)
Time (min)
Control (Dry)
Cold Water
Warm Water
24 Hr.
48 Hr.
0
2170
2183
2168
2166
1667
1
2114
2217
2291
2227
1916
2
2094
2240
2291
2320
2143
3
2094
2236
2326
2404
2363
4
2090
2254
2380
2466
2520
5
2094
2266
2437
2540
2855
Total Change (0-5 min)
-76 ppm
+83 ppm
+269 ppm
+374 ppm
+1,188 ppm
Alaskan Peas
CO2 ppm (parts per minute)
Time
Control (Dry)
Cold Water
Warm Water
24 Hr.
0
2200
1991
1850
2299
1
2060
2022
2021
2759
2
2051
2012
2095
2735
3
2024
2030
2195
2865
4
2024
2063
2302
3138
5
2024
2113
2387
3355
Total Change (0-5 min)
-176 ppm
+122 ppm
+537 ppm
+1,056 ppm
Analysis: While observing the data, the change in CO2 ppm for the control (dry) peas/beans are both moving at a constant rate.
Due to the control, non germinating peas/beans are releasing less CO2 ppm than the 24 hour and 48 hour germinating peas/beans. This can be seen when the control from 0-5 minutes was at -76 ppm, while the 24 hour beans were at +374 ppm. The alaskan peas also saw this same type of pattern from 0-5 minutes. The control for the peas was at -176 ppm and the germinating peas were at +1,056 ppm. Since this lab was conducted with precision, and the sensors were not broken, or tampered with before, during or after the experiment, this data is accurate which leads to precise
calculations.
Conclusion: The results of this lab dp support the hypothesis which is “The germinating beans, and peas will discharge more CO2 than the beans and peas controls.” Since the germinating beans and peas produced more CO2 than the beans/peas that were dry and had not germinated in any water. The reason behind the control not releasing any CO2 because the process called germination occurs when a seed has been soaked in water and has begun releasing CO2 due to the addition of H2O. Seeds that are not soaked within the water are in a dormant state, which leads to the beans/peas releasing less CO2 because no H2O is involved with the process.
Bibliography: "Cellular Respiration." Web. 29 Nov. 2014. . “AP Biology.” Book. 29 Nov .2014..