Glucose Challenge Lab Report

Glucose challenge was conducted through intravenous application of 50% glucose (0.3 g/kg of body weight) in 1 min periods in the antecubital vein. The clock was restarted at the beginning of glucose application. Samples used for measuring insulin and c-peptides were taken precisely in +2 min, +4 min, +6 min and +8 min after initiation of glucose administration. Samples for measuring glycemia were taken in +2 min, +4 min, +6 min and +8 min. Upon completed sampling, insulin infusion was stopped, while glucose infusion was continued, with gradually reduced speed and giving food to subject, accompanied by monitoring of glycemia. Glycemia had to be higher than 5 mmol/l, and stable for 30 min upon completing glucose infusion. Acute insulin response (AIR) level was calculated, which is actually average increase of insulinemia relative to basal value in the first 8 min of IVGTT, with the following equation:
AIR= ((insulinemia u +2 min (mU/L)-insulinemia u 0 min (mU/l))+ (insulinemia u +4 min (mU/L)-insulinemia u 0 min (mU/l))+ (insulinemia u +6 min (mU/L)-insulinemia u 0 min (mU/l))+ ((insulinemia u +8 min (mU/L)-insulinemia u 0 min (mU/l))/4

First phase of insulins response (FPIR) is calculated using following equation:

FPIR= insulinemia u +2 min (mU/l) + insulinemia u +4 min