Preview

Glucose Oxidase

Good Essays
Open Document
Open Document
628 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Glucose Oxidase
Glucose Oxidase and Its Various Uses

Aaron Truong

Since glucose oxidase has an end product called hydrogen peroxide, which is a harmful substance to bacteria, it can be used to fight bacteria, or sterilize objects (can have various uses such as in hand sanitizers, toothpaste, soap, etc), not just biosensors.

Another key part in the reaction would be C6H12O6, or glucose. Glucose oxidase can be applied to diabetics as mentioned earlier, as biosensors work by "keeping track of the number of electrons that pass through the enzyme by connecting it to an electrode and measuring the resultant charge" (From Biosensors to Food Preservative). This would allow biosensors to monitor blood glucose levels, improving the lives of diabetics. (From Biosensor to Food Preservative)

In the world today, enzymes are used in a variety of industries. They can be used to preserve food, to fight bacteria and illnesses, or to manufacture products that will benefit those in need. An enzyme that is almost hailed as a valuable gold mine today would be glucose oxidase. In the world of diabetics, it is a health problem that affects millions of people. With careful food management, the optimum blood glucose levels can be maintained. One significant use of glucose oxidase is to estimate the amount of glucose in a certain part, and has been applied to biosensors, making life easier for diabetics. It can be applied to used in "chemical, pharmaceutical, food, beverage, clinical chemistry, biotechnology and other industries" (US National Library of Medicine, Glucose oxidase--an overview)

In the pharmaceutical industry, glucose oxidase was discovered to be an antibiotic during the 1920's to the 1940's from a study of anti-bacterial properties in Penicillium fungi. A purer state of Notatin, which was also derived from Penicillium fungi, it was shown to have a high specificity for glucose, which was oxidized to gluconic acid. Its reaction equation would be:
C6H1206 + 02 +H20

You May Also Find These Documents Helpful

  • Good Essays

    Glucose exposure resulted in a yellow color change proving that Unknown #7 fermented glucose and produced an acid but when exposed to Sucrose Unknown #7 produced a red color change indicating that it was a non-fermented of sucrose which was reflected with an alkaline bi-product. The Enterotubes showed that Unknown #7 was an "enteric" bacterium with a score of "46141" Lysine, Adonitol, Lactose, Dulcitol/Phenylalanine, and Harnstoff/Uree were all negative, while Ornithine, Arabinose, and Sorbitol were proven to be positive. The IMViC test identifies members of Enterobacteriaceae and included Indole that tested negative, Methyl Red which produced a red color change showing that Unknown #7 could ferment glucose to acidic ends, Vogves Proskaer which tested negative because Unknown #7 failed to convert those acidic bi-products back to neutral ends, and Citrate which tested positive with a blue color change and growth on the medium because Unknown #7 was able utilize citrate. The Nitrate Reduction test displayed Incomplete Reduction with a red color change when Solution A and B were added meaning that it could only reduce NO3- to NO2-…

    • 553 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Lab Report On Unknown 32

    • 591 Words
    • 3 Pages

    When unknown 32 was tested with the oxidase test, it was found oxidase negative. The catalase test tests for the presence of catalase which breaks down hydrogen peroxide and oxidase dismutase. A small amount of hydrogen peroxide is dropped onto a slide of unknown 32 and resulted in bubbles. This result shows that unknown 32 is catalase positive since it broke down hydrogen peroxide and oxidase dismutase which resulted in water and oxygen. This eliminated Enterococcus spp.…

    • 591 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    BIOS242 W4 Lab Template

    • 460 Words
    • 3 Pages

    o All four bacteria were able to metabolize glucose, but not all produced identical results. Predict the metabolic products expected for the four bacteria. A chemical energy is released when glucose is broken down. Glucose is broken down into CO2and H2O. A glucose test is screening the blood for signs of high blood glucose and low blood glucose. Not only not, but this test can help diagnose diabetes as well.…

    • 460 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Bio Lab Essay K101

    • 1732 Words
    • 7 Pages

    The main objective of this lab was to observe the activity of enzyme peroxidase in real time under different experimental conditions. To see how peroxidase reacts with its substrate guaiacol under different conditions. We measure the amount of substances per test tube and then combine all of them together. Each test tube contains a measured amount of any listed substances including H₂O. Each measured amount of peroxidase along with its substrate guaiacol, and other listed substances will show how actively the enzyme oxidizes the substrate. To measure the…

    • 1732 Words
    • 7 Pages
    Good Essays
  • Satisfactory Essays

    The enzyme used in this experiment is Invertase. This enzyme catalyzes the breakdown of sucrose (table sugar) into glucose and fructose.…

    • 704 Words
    • 8 Pages
    Satisfactory Essays
  • Satisfactory Essays

    3. Catalase is an antioxidant enzyme, which is naturally produced in most organisms, used to help break down hydrogen peroxide into oxygen and water. The break down of hydrogen peroxide helps prevent the accumulation of carbon dioxide bubbles in the blood. It is considered to have a great impact because it is capable of decomposing millions of hydrogen peroxide molecules. In addition, it uses the hydrogen peroxide to oxidize harmful toxins within organisms, such as formaldehyde, alcohol, and phenol.…

    • 315 Words
    • 1 Page
    Satisfactory Essays
  • Good Essays

    Natural enzymes are proteins that catalyze biological reactions by lowering the activation energy of the reaction without being altered during the process. The enzyme used in this experiment was the β-galactosidase purified from E. coli. This enzyme hydrolyzes lactose and turns it into galactose and glucose. Since it is difficult to assay the activity of β-galactosidase, we will be using the artificial substrate, o-nitrophenyl-β-galactoside (ONPG) instead of lactose. ONPG is an analog of lactose and an advantage of using ONPG is that it is easy to determine the amount of ONPG cleaved by using spectrometric assay (1). The β-galactosidase hydrolyzes ONPG and yields a yellow solution that contains o-nitrophenol and galactose. The solution becomes more yellow as the more ONPG is being degraded. Using spectrophotometry, the absorbance of the solution can be determined at a wavelength of 420nm. The assays will help determine the Km, Vmax, and Kcat of the enzyme. In our assays, Na2CO3 is used to stop the reactions by changing the solution pH to basic and as a result the enzyme will become inactive.…

    • 463 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Catalase Lab Report

    • 563 Words
    • 3 Pages

    Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach), Brassica oleracea (Broccoli), Solanum tuberosum (Russet Potato), Malus domestica (Apple), and Allium cepa (Onion). The five different catalases from the sources will all be used to catalyze Hydrogen peroxide (H2O2). When hydrogen…

    • 563 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Unknown Lab Report

    • 2145 Words
    • 9 Pages

    The oxidase test was performed only on gram (-) bacteria and was used to test for the presence of cytochrome oxidase. Living bacteria were placed on a paper towel and saturated with a chromogenic reducing agent. Within seconds the reagent, acting as an artificial electron acceptor, will turn purple if oxidized cytochrome oxidase is present, indicating a positive test. If no color change is observed, no cytochrome oxidase is present and the test is negative.…

    • 2145 Words
    • 9 Pages
    Better Essays
  • Good Essays

    Biology: Key Questions

    • 665 Words
    • 3 Pages

    This process aids us in controlling blood sugar, by the hydrolysis of glycogen to glucose.…

    • 665 Words
    • 3 Pages
    Good Essays
  • Better Essays

    amylase lab report

    • 1766 Words
    • 8 Pages

    There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions, such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva, and pancreatic secretions of the small intestine. The function of amylase is to break down big molecules of starch into small molecules like glucose; this process is called hydrolysis. Enzymes are very specific; for example, amylase is the only enzyme that will break down starch. It is similar to the theory of the lock and the key. The enzyme is the lock and the key is the substrate; only the correct key could fit into the keyhole of the lock.…

    • 1766 Words
    • 8 Pages
    Better Essays
  • Satisfactory Essays

    The intense roaring of the crowd gradually faded into the vast abyss of his mind. As the last seconds ticked away, his breath got heavier and he knew that it was now or never. He knew he had to make the shot, he could not just mess this one up. At that last moment when the ball left his hands flying into the air, time seemed to have slowed down as the buzzer went off simultaneously.…

    • 173 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    The enzyme in this experiment was catalase, which was extracted from potato and liver. The reactant in this experiment was hydrogen peroxide and it was added to the enzyme after the enzyme was put through different environments.…

    • 575 Words
    • 3 Pages
    Satisfactory Essays
  • Better Essays

    Peroxidase

    • 1567 Words
    • 7 Pages

    The term peroxidase refers to both a class of oxidoreductase enzymes and to specific enzymes within that class. As a general class of enzymes, peroxidases catalyze the oxidation−reduction decomposition reaction of hydrogen peroxide. There are two general types of peroxidases—catalase and peroxidase. Catalase catalyzes the disproportionation reaction of hydrogen peroxide to water and oxygen gas (Equation 1). In reactions mediated by catalase, hydrogen peroxide substrate molecules act as both oxidizing agent (electron acceptor) and reducing agent (electron donor). In contrast, peroxidase acts in the presence of other naturally occurring organic reducing agents, such as ascorbic acid and glutathione, to catalyze the decomposition of hydrogen peroxide. Organic reducing agents, abbreviated AH2, transfer hydrogen atoms and electrons to hydrogen peroxide, resulting in the formation of water and oxidized organic substrates such as A2 in Equation 2. Catalase-catalyzed reaction Peroxidase-catalyzed reaction 2H2O2 → 2H2O + O2 2H2O2 + 2AH2 → 4H2O + A2 Equation 1 Equation 2…

    • 1567 Words
    • 7 Pages
    Better Essays
  • Better Essays

    Catalase

    • 2435 Words
    • 10 Pages

    Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in reproductive reactions. Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of molecules of hydrogen peroxide to water and oxygen each second. Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four porphyrin heme (iron) groups that allow the enzyme to react with the hydrogen peroxide. The optimum pH for human catalase is approximately 7, and has a fairly broad maximum (the rate of reaction does not change appreciably at pHs between 6.8 and 7.5). The pH optimum for other catalases varies between 4 and 11 depending on the species. The optimum temperature also varies by species.…

    • 2435 Words
    • 10 Pages
    Better Essays