Golgi Apparatus Assignment
Biology Assignment: Golgi
Apparatus
Contents
• Introduction to Golgi Apparatus.
• Importance of Golgi Apparatus in Cell.
• Techniques involved in study of Golgi Apparatus.
• Diseases Related to Golgi Apparatus.
• Economical Relevance of Golgi Apparatus.
• Treatment of the diseases related to Golgi
Apparatus.
Introduction to Golgi Apparatus
• Discovered by “Camillo Golgi” in 1898 during investigation of nervous system.
• Works as an independent organelle.
• Golgi apparatus is present in eukaryotic cells as one or more groups of flattened, membrane-bounded compartments or sacs. • Golgi apparatus is responsible for the processing and packaging of protein and lipids.
• The cisternae stack has four functional regions: the cis-Golgi network ,medal-Golgi, endo-golgi, and trans-Golgi network. Vesicles from the endoplasmic reticulum.
Location of Golgi
Apparatus in cell.
Working of Golgi
Apparatus.
Techniques used to study the Golgi Apparatus.
• Cell Staining: Fluorescent Labelling of the
Golgi Apparatus, Immunofluorescence can be used to visualize proteins in the Golgi apparatus or its subdomains.
• Immunofluorescence has been the primary method for labelling the Golgi apparatus for light microscopic observation in fixed cells.
• With the common availability of the green fluorescent protein (GFP) and advanced fluorescence microscopes including confocal microscopes, imaging of the Golgi apparatus in living cells is now a major experimental approach. • With the development of new specimen preparation techniques and improvements in instrumental resolution, scanning electron microscopy (SEM) became an effective means of studying the three-dimensional organization of the Golgi complex.
• All Golgi Complex cisternae displayed uniform PA-TCH-SP staining. Diseases Related to Golgi
Apparatus.
Achondrogenesis
Achondrogenesis type 1A is caused by a defect in the microtubules of the Golgi
Apparatus
Contents
• Introduction to Golgi Apparatus.
• Importance of Golgi Apparatus in Cell.
• Techniques involved in study of Golgi Apparatus.
• Diseases Related to Golgi Apparatus.
• Economical Relevance of Golgi Apparatus.
• Treatment of the diseases related to Golgi
Apparatus.
Introduction to Golgi Apparatus
• Discovered by “Camillo Golgi” in 1898 during investigation of nervous system.
• Works as an independent organelle.
• Golgi apparatus is present in eukaryotic cells as one or more groups of flattened, membrane-bounded compartments or sacs. • Golgi apparatus is responsible for the processing and packaging of protein and lipids.
• The cisternae stack has four functional regions: the cis-Golgi network ,medal-Golgi, endo-golgi, and trans-Golgi network. Vesicles from the endoplasmic reticulum.
Location of Golgi
Apparatus in cell.
Working of Golgi
Apparatus.
Techniques used to study the Golgi Apparatus.
• Cell Staining: Fluorescent Labelling of the
Golgi Apparatus, Immunofluorescence can be used to visualize proteins in the Golgi apparatus or its subdomains.
• Immunofluorescence has been the primary method for labelling the Golgi apparatus for light microscopic observation in fixed cells.
• With the common availability of the green fluorescent protein (GFP) and advanced fluorescence microscopes including confocal microscopes, imaging of the Golgi apparatus in living cells is now a major experimental approach. • With the development of new specimen preparation techniques and improvements in instrumental resolution, scanning electron microscopy (SEM) became an effective means of studying the three-dimensional organization of the Golgi complex.
• All Golgi Complex cisternae displayed uniform PA-TCH-SP staining. Diseases Related to Golgi
Apparatus.
Achondrogenesis
Achondrogenesis type 1A is caused by a defect in the microtubules of the Golgi