Growth Curve Report
GROWTH CURVE
NAME SURNAME :ahmet mehmeh
STUDENT ID :
DEPARTMENT : MOLECULAR BIOLOGY AND GENETİCS
DATE OF EXPERIMENT: 02.03.2011
ABSTRACT
In this experiment,the cell growth of yeast is measured by using spectrophotometer and hemocytometer.We learnt how specthophotometer and hemocytometer use and also we learnt qualifications of hemocytometer and spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution,we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth curve.
INTRODUCTION
In this experiment,we learnt cell growth.Cell growth means increase in the quantity of cellular constituents.Cells in culture double its number because they produce two daughter cells by dividing.Generation time is the constant time when cells in the culture double their number.This kind of growth depends on some requirements such as type of the cell and environment.Two different parameters can be used to calculate growth for unicellular organisms such as bacteria and yeast:changes in cell mass and cell number.There are some different ways for measuring the cell growth with cell mass.Physical measurement:dry weigth,wet weigth or volume after centrifuge and: Chemical measurement chemical components such as N,total protein and DNA and Chemical activity:rate of , CO2 production or consumption; Turbidity (spectrophotometric) measurement: based on turbidity and measure both dead and alive cells.Spectrophotometer can measure intensity of the light source wavelength.Spectrophotometers are important in terms of spectral bandwith and linear range of absorption or reflectance measurement.Spectrophotometers are used for physics,material science,chemistry,biochemistry and molecular biology experiments.Why must spectrophotometerbe set to
NAME SURNAME :ahmet mehmeh
STUDENT ID :
DEPARTMENT : MOLECULAR BIOLOGY AND GENETİCS
DATE OF EXPERIMENT: 02.03.2011
ABSTRACT
In this experiment,the cell growth of yeast is measured by using spectrophotometer and hemocytometer.We learnt how specthophotometer and hemocytometer use and also we learnt qualifications of hemocytometer and spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution,we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth curve.
INTRODUCTION
In this experiment,we learnt cell growth.Cell growth means increase in the quantity of cellular constituents.Cells in culture double its number because they produce two daughter cells by dividing.Generation time is the constant time when cells in the culture double their number.This kind of growth depends on some requirements such as type of the cell and environment.Two different parameters can be used to calculate growth for unicellular organisms such as bacteria and yeast:changes in cell mass and cell number.There are some different ways for measuring the cell growth with cell mass.Physical measurement:dry weigth,wet weigth or volume after centrifuge and: Chemical measurement chemical components such as N,total protein and DNA and Chemical activity:rate of , CO2 production or consumption; Turbidity (spectrophotometric) measurement: based on turbidity and measure both dead and alive cells.Spectrophotometer can measure intensity of the light source wavelength.Spectrophotometers are important in terms of spectral bandwith and linear range of absorption or reflectance measurement.Spectrophotometers are used for physics,material science,chemistry,biochemistry and molecular biology experiments.Why must spectrophotometerbe set to
References: * file:///C:/Documents%20and%20Settings/Admin/Desktop/Spectrophotometry.htm * file:///C:/Documents%20and%20Settings/Admin/Desktop/Serial%20Dilutions.htm * http://www.wisegeek.com/what-is-cell-viability.htm file:///C:/Documents%20and%20Settings/Admin/Desktop/The%20Methylene%20Blue%20Staining%20Procedure%20and%20Yeast%20Viability%20-%20Associated%20Content%20-%20associatedcontent.com.htm http://www.cuvetteshop.com/ http://en.wikipedia.org/wiki/Spectrophotometry http://www.gmi-inc.com/AnyLab/HP%208452A%20Spectrophotometer.htm http://www.cellsalive.com/ecoli.htm http://www.umsl.edu/~microbes/pdf/introductiontobacteria.pdf