HPTLC Flavonoid Profile Of Methanol Extract M. Concanensis Case Study

A- HPTLC Flavonoid profile of methanol extract M. concanensis leaves
B- HPTLC Flavonoid profile of methanol extract M. concanensis flowers
C- HPTLC Flavonoid profile of methanol extract M. concanensis seeds

4.2.3.1 C) HPTLC Phenol profile of M. concanensis Nimmo.

HPTLC finger printing of M. concanensis was done by using selected solvent system Chloroform: Ethyl acetate: Formic acid (50%:40%:10%v/v) for leaf, flower and seed extracts, visualized under UV 254 and 366 nm showed more components. The Rf values and chromatogram in the methanol extract of leaf, flower and the seed was presented in the Table-8. In general, more degree of phyto compounds diversity has been observed in vegetative part when compared with the vegetative parts. The methanol extract of leaves, flowers and seeds of M. concanensis showed the presence of 33 peaks with different types of Rf values with range from 0.01 to 1.04. A total number of 6 peaks with Rf values 0.01, 0.41, 0.47, 0.73, 0.84 and 1.04 for leaves, 13 peaks with Rf values 0.11, 0.26, 0.40, 0.49, 0.54, 0.69, 0.71, 0.78, 0.84, 0.92, 0.96, 0.98 and 1.02 for flower and 14 peaks with Rf value 0.03, 0.26, 0.35, 0.40, 0.53, 0.55, 0.62, 0.71, 0.77, 0.85, 0.93, 0.95, 1.00 and 1.03 were observed in seeds. The maximum phenol content of M. concanensis was observed in leaves followed by flowers and seeds. This is in harmony with phenols standard of Gallic acid, the Rf value being 0.85 present in the seed extract showed in the plate-19.
concanensis Nimmo.

S.No. Rf A B C Gallic acid
1 0.01 +
2 0.03 +
3 0.08
4 0.11 +
5 0.14
6 0.26 + +
7 0.30
8 0.35 +
9 0.40 + +
10 0.41