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Determine the presence of catalase enzyme activity using hydrogen peroxide.
1. Test for the presence of catalase in liver.
A. Using a knife, cut a slice of liver that measures approximately 1 cm by 1 cm and place it on a slide.
B. Using a micropipette, place a few drops of hydrogen peroxide (H2O2) on top of the liver.
C. Observe the solution for a possible reaction, indicated by bubbling. Record the results.
2. Test for the presence of catalase in lunch meat.
A. Cut a piece of roast beef lunch meat approximately 1 cm by 1 cm and place it on a slide.
B. Using the pipette, place a few drops of hydrogen peroxide (H2O2) on top of the lunch meat.
C. On the table provided below, record the results.
D. Repeat the steps for 2 above with a lunch meat of the student's choosing. (Do not use roast beef.)
Measuring pH Activity
1. Use the five plastic cups from the previous activity.
2. Label the first cup "vinegar." Label the second cup "shampoo," a third "lemon juice," a fourth "water," and the fifth "baking soda."
3. Using a measuring spoon, add 1 teaspoon of baking soda to the cup labeled baking soda.
4. Using the graduated cylinder, measure out about 10 mL of distilled water. Pour the water from the cylinder into the cup labeled baking soda and stir until the baking soda dissolves. It may be necessary to adjust the amount of water, adding more, if needed until the baking soda is completely dissolved. Alternatively, if the baking soda dissolves readily, the student may need to add more baking soda.
5. Dip a new pH strip into the cup.
6. Compare the color of the pH strip to the color chart included with the strip and record the pH.
7. Pour a small amount of vinegar into the corresponding cup.
8. Repeat Steps 5 and 6.
9. Repeat Steps 7 and 8 for each
Determine the presence of catalase enzyme activity using hydrogen peroxide.
1. Test for the presence of catalase in liver.
A. Using a knife, cut a slice of liver that measures approximately 1 cm by 1 cm and place it on a slide.
B. Using a micropipette, place a few drops of hydrogen peroxide (H2O2) on top of the liver.
C. Observe the solution for a possible reaction, indicated by bubbling. Record the results.
2. Test for the presence of catalase in lunch meat.
A. Cut a piece of roast beef lunch meat approximately 1 cm by 1 cm and place it on a slide.
B. Using the pipette, place a few drops of hydrogen peroxide (H2O2) on top of the lunch meat.
C. On the table provided below, record the results.
D. Repeat the steps for 2 above with a lunch meat of the student's choosing. (Do not use roast beef.)
Measuring pH Activity
1. Use the five plastic cups from the previous activity.
2. Label the first cup "vinegar." Label the second cup "shampoo," a third "lemon juice," a fourth "water," and the fifth "baking soda."
3. Using a measuring spoon, add 1 teaspoon of baking soda to the cup labeled baking soda.
4. Using the graduated cylinder, measure out about 10 mL of distilled water. Pour the water from the cylinder into the cup labeled baking soda and stir until the baking soda dissolves. It may be necessary to adjust the amount of water, adding more, if needed until the baking soda is completely dissolved. Alternatively, if the baking soda dissolves readily, the student may need to add more baking soda.
5. Dip a new pH strip into the cup.
6. Compare the color of the pH strip to the color chart included with the strip and record the pH.
7. Pour a small amount of vinegar into the corresponding cup.
8. Repeat Steps 5 and 6.
9. Repeat Steps 7 and 8 for each