-Hygienic measures reduce the numbers of resident & transient microorganisms & the likelihood of infection…
Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media, including MRS broth and nutrient broth, learn about oxygen and temperature requirements for microbial growth and how to control microbial growth.…
Purpose: The purpose of this experiment is to Learn and employ aseptic technique, to become familiar with basic requirements of microbial growth, to learn the basic forms of culture media, and to become familiar with methods used to control microbial growth.…
One of the discs was supposed to have a rather large inhibition zone and instead it was very small. Because of this, we question if it was placed right, and again, it changed the data we were able to collect. The last source of uncertainty for our experiment was how long the plates were uncovered in the swabbing and disc application process. If they were left uncovered for an uncertain amount of time, it would impact how the E. coli bacteria would have grown on the plate. In addition to that, if there were some other sort of air born microbe that could have landed on the plate, it could impact how the E. coli would…
Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time, propose is to isolate the unknown bacteria. Therefore, the first time to streak on the plate, there are million of bacteria on the loop. For that reason, we need to sterilize the loop before next streaking. Then we can get small group of colonies out from the large group of colonies to observe and distinguish the unknown bacteria.…
The media was inoculated with a single stab to the center of the tube and incubated. After incubation, the growth was restricted to the stab line, the bacteria is nonmotile.…
On the control plate, no observable growth of the microbes was experienced. Otherwise, if there could have been any noticeable growth on this plate, then the aseptic techniques applied were not effective enough to prevent accidental introduction of microbes through contamination. In addition, the control experiment ought…
References: 1. Sigmon, J. Bio 225: Microbiology Lab Manual, York Technical College, 2004, pp. 1-58…
The statistical analysis displayed by text notations in Table 1 shows that the p-value of the difference in treatment is greater than 0.05 (defined as p-value>0.05). This suggests that the difference between resulting colonies per treatment per bacteria is significant. Through this, it is determined that the photodynamic inactivation of gram-negative bacteria E. coli colonies is significantly more effective than the inactivation of gram-positive bacteria S. aureus colonies. In table 3, the survival fraction (SF) and the growth inhibition by percentage (%) were calculated from the mean CFU miliLiter-1 of each treatment per bacteria to further determine the trend of the treatments applied.…
For how wide spread of a sampling of bacteria we obtained, the actual colony observations didn't differ drastically. All samples, including the control, were in and around the same color spectrum in lightish brown coloring. The control, stair railing and neath of the fingernail were all circular in form and the margin for those three was also entire and all elevations were convex. The diameter of the actual colonies was where the most diverse observations occurred. The percentage varied from one to fifty percent and the millimetre size also varied from less than 2mm to about 5mm.…
The great-horned owl is one of the most mysterious animals of the world. In an excerpt from Mary Oliver's essay "Owls," she discusses her fear as well as her utmost admiration of this most frightening of creatures.…
The article titled, “ ‘Harmless’ Things You Should Really Wash Your Hands After Touching” by William Harris, is a mind blowing article that shows how every day of people's lives they get germs from all the things they touch and how it can affect them. The article titled, “ ‘Harmless’ Things You Should Really Wash Your Hands After Touching” by William Harris, is a mind blowing article that shows how every day of people's lives they get germs from all the things they touch and how it can affect them. According to the author of this article, William Harris, he claims that when people come in contact with any array of fomites ,they are surfaces that are flooded with bacteria, these surfaces would give people a bigger of a chance to get diseased…
Florence Nightingale, was the first women that is recognized with founding modern nursing and creating the first educational system for nurses. (Creasio, Friberg 2011) The nursing profession has improved considerably over the years with different educational opportunities that individuals can choose from. There is a clear difference in competency levels between the Associate degree and the Baccalaureate degree (BSN) in nursing. With this being said, the BSN degree nurse provides more leadership skills, critical…
(ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar, CLED agar and MacConkey agar; the number of species present also had increased in growth.…
The society is grateful to the support of the UP Interactive Learning Center, the Globe…