The experiment was done in three parts: firstly the separation of sucrose, then the separation of Aspirin and the isolation of the unknown component.…
Enzymes are biological catalysts. They work by lowering the activation energy needed to initiate a chemical reaction. Enzymes work within an optimal temperature and optimal pH. Enzymes are highly specific for a single substrate. The Enzyme is usually much larger in size than the substrate it binds to. In some cases, an enzyme requires something called a cofactor to begin the chemical reaction. There were four different experiments that were executed in the enzyme lab. Experiment 7.1, the first experiment, was performed to test the effect of temperature on enzymatic activity. Based on what I know about the effect of temperature on the enzymatic activity of lactase, if the lactase used in today’s lab was extracted from human cells, I hypothesize that the optimal temperature for lactase to be in is around 37 degrees Celsius, which is the average human body temperature. The second experiment performed, experiment 7.2, tested the effect of pH on enzymatic activity. Based on what I know about the effect of pH on the enzymatic activity of lactase, if the lactase used in today’s lab was extracted from human cells, I hypothesize that the optimal pH level is 7, which is the average pH level in humans. Experiment 7.3 tests enzymatic specificity. Knowing that lactase is specific for the substrate lactose, lactase will only bind to lactose and not work with the other substrate used in this experiment, maltose. The last experiment of the lab, 7.4, was done to determine the cofactors of the enzyme, lactase. I hypothesize that EDTA, a cofactor inhibitor, will have a negative effect on the reaction and lower the amount of glucose that could possibly be produced if EDTA was not present.…
For this experiment our dependent variable was the paper with yeast on it, and our independent variable was the concentration of Hydrogen Peroxide we put in each solution. We tested the amount of time it took for the peroxide to break down yeast at 3%, 2.25%, 1.5%, .75% and 0% concentration. The fastest reaction time was 1.7 at 3% concentration. We…
The aim of this experiment was to find the favoured temperature for types of micro-organisms to grow. The hypothesis was sort of rejected in this experiment as our rule of thumb didn’t add up to the results we got. If we could make our results more accurate, we could have supported our hypothesis but as the results were inaccurate, we could not prove that our hypothesis was correct. Overall this experiment went well even though there were some difficulties and problems that occurred which altered our…
Analysis Question: I would have tried to control the experiment more by trying different methods such as temperatures and amount of Alka-Seltzer…
statement of problem: in this lab we will determine how enzymes affect the amount of apple juice produced from applesauce we will test pectinase cellulase and a combination of both of of the enzymes and then finally we will use water ( which will be used as the controlled variable).…
The purpose of this lab is to answer the research question: “Does the concentration of sucrose affect the rate of cellular respiration in yeast?” What do you think?…
Investigation Followup PROCEDURE My investigation was what what would happen to gummy bears when they were put into different solutions, if they would get bigger, smaller, or stay the same. Such as would it change color, texture, and/or weight. My hypothesis was that the gummy bears would get bigger in all the solutions, they would get bigger in the forms of length, width, and height.…
: The purpose of this lab is to observe and explore the relationship of a substance’s…
At MEND Poverty Clinic, I was surprised by how many patients do not realize that their bad habits can cause a permanent condition like periodontal disease. Lack of awareness of oral health has affected the health of my people in this community. After treating many unfortunate patients, such as a seventeen-year-old patient that needed extractions and only had few teeth left, an implant program was implemented which I am in charge with Dr. Robert Hale in order to improve the life of many…
In this lab activity students performed an experiment with the use of distilled water and baking soda solutions as control. The experimental controls are accuracy indicators of laboratory results. Thus, controls are important in a lab because the observation results are dependent on the controls. The experimental results can be determined to be valid when it is compared with the control solution. The higher the similarity between the results and the control solution, the more accurate the investigation is.…
Our class began a lab based around enzymes and how they react when different variables are changed, such as temperature, pH, and concentration of the yeast or hydrogen peroxide. The yeast acted as the enzyme, which produces catalase needed for our desired reaction with the hydrogen peroxide. What had to be wanted to measure was how well it reacted when the variables were…
The experiment could be improved by doing one test tube at a time. This will improve the test because it will give you most exact results because the scientist will be able to focus more on that one test tube and make every move as perfect and exact as possible. Another way to improve your experiment could be to clean out test tubes better before using it for the experiment. The class before was using the same test tube and might not have washed them out well enough. If the test tubes still have ingredients in them, it may affect the way that the fermentation occurs whether it be improving the rate or decreasing the rate of fermentation. Either way, the rate could be changed causing the results to not be accurate. If a baker was trying to achieve very fluffy bread, the baker should include baking powder, because when reacted with the sugar water and yeast, it produces a high rate of fermentation. If a baker wants to avoid ingredients that may decrease the fluffiness in bread, the baker would want to limit the amount of salt and baking soda, because it decreases the amount of fermentation that…
Note that formally observations and results or explanations for the observations are treated separately in a laboratory report. Since these experiments are rather simple, we will note our observations and…
1. Number of potato cubes: 3 cubes of potatoes were used in each trial at each different temp. If changed, whether decrease or increase, then the number of enzymes…