How Does Ph Affect Enzyme Activity
Factors affecting Enzyme Activity:
The effect of pH on enzyme activity
Background Knowledge:
An enzyme is a biological catalyst – which speeds up the reaction rate, without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme. Substrate ------enzyme -----> Product
Extremities of pH affects the activity of an enzyme. The extremities of pH permanently alter the shape of the enzyme and even its active site, thus making the enzyme inactive ,i.e. enzyme gets denatured .1The enzyme used in this experiment is catalase and is found in …show more content…
Hypothesis: An enzyme works the best at an optimum ph. The rate of enzyme activity increases with the increase in the pH, until it reaches an optimum pH, at which the enzyme exhibits the maximum activity. The rate of activity then decreases with the increase in the pH of the medium. In this case ,the varying height of the foam at different pHs tell us the rate of activity for a time interval.
Independent Variable:
➢ pHs of the buffer medium.
Dependent Variable:
➢ The amount of foam formed in the test tube (ml).
Control Variables:
➢ The amount of distilled water and buffer of different pHs.
➢ Temperature of water bath at 25 oC : Differing temperature affects the rate of activity of an enzyme.
➢ Test tube: Using the same type of test tube for all the trials.
➢ Time interval: The amount / length of foam formed in 45 seconds using a stop watch recorded in cm
Materials Required:
1. Catalase Enzyme - 20 grams.
2. Measuring Test tube - 5 to 7.
3. Beaker - 2.
4. Distilled Water - 500
The effect of pH on enzyme activity
Background Knowledge:
An enzyme is a biological catalyst – which speeds up the reaction rate, without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme. Substrate ------enzyme -----> Product
Extremities of pH affects the activity of an enzyme. The extremities of pH permanently alter the shape of the enzyme and even its active site, thus making the enzyme inactive ,i.e. enzyme gets denatured .1The enzyme used in this experiment is catalase and is found in …show more content…
Hypothesis: An enzyme works the best at an optimum ph. The rate of enzyme activity increases with the increase in the pH, until it reaches an optimum pH, at which the enzyme exhibits the maximum activity. The rate of activity then decreases with the increase in the pH of the medium. In this case ,the varying height of the foam at different pHs tell us the rate of activity for a time interval.
Independent Variable:
➢ pHs of the buffer medium.
Dependent Variable:
➢ The amount of foam formed in the test tube (ml).
Control Variables:
➢ The amount of distilled water and buffer of different pHs.
➢ Temperature of water bath at 25 oC : Differing temperature affects the rate of activity of an enzyme.
➢ Test tube: Using the same type of test tube for all the trials.
➢ Time interval: The amount / length of foam formed in 45 seconds using a stop watch recorded in cm
Materials Required:
1. Catalase Enzyme - 20 grams.
2. Measuring Test tube - 5 to 7.
3. Beaker - 2.
4. Distilled Water - 500