How does ph affect enzyme reactivity
Observing Enzyme Activity
Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate.
Hypothesis: If the enzyme is in a basic solution, then it will react faster because the enzyme (catalase) reacts better in basic solutions.
Materials:
10 potato cubes (1 cm3) -Pipet
Baking soda solution -50 ml glass beaker
Bleach
Water
Lemon juice
Vinegar
5 glass test tubes
Drying rack
Timer
Graduated cylinder
Hydrogen peroxide
Procedure:
1. Place all 5 glass tubes face up in the drying rack
2. Use tweezers and soak one potato cube into water and leave it there for 2 minutes.
3. After 2 minutes, use tweezers and place the potato into the first testing tube.
4. Repeat steps 2 and 3 four more times except soaking the other 4 potatoes in baking soda solution, bleach, vinegar, and lemon juice. Place the potatoes in each tube respectively.
5. Pour hydrogen peroxide into the 50 ml glass beaker. (about 25 ml)
6. Use the pipet and measure up 5 ml of hydrogen peroxide in the graduated cylinder
7. Pour the 5 ml of hydrogen peroxide into the first test tube. Start the timer immediately. Time for 7 minutes. Observe and record.
8. Repeat steps 6 and 7 with the other 4 potatoes.
9. Repeat steps 2-8 twice.
Data
Test Tube
Height of foam after 7mm
Color of the Solution
Observations
1(Water)
1mm
Clear
Fizzes, Bubbles
2(Lemon Juice)
0mm
Clear
Small fizzing, not too violent
3(Vinegar)
0mm
Clear
Nothing happens to it.
4(Baking Soda)
4mm
Neon, yellow, but still clear.
Very active, light foam.
5(Bleach)
5mm
Pale yellow
Foamy, lots of bubbles, white dense foam. (exact same results for both trials)
Results
My results showed bleach having the highest amount of foam 5mm, baking soda with the 2nd highest amount of foam (4mm), water being the third (1mm) and lemon juice and vinegar being last at (0mm). Bleach which was first had the ph of 13, baking soda with the ph of 9, water with the ph of
Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate.
Hypothesis: If the enzyme is in a basic solution, then it will react faster because the enzyme (catalase) reacts better in basic solutions.
Materials:
10 potato cubes (1 cm3) -Pipet
Baking soda solution -50 ml glass beaker
Bleach
Water
Lemon juice
Vinegar
5 glass test tubes
Drying rack
Timer
Graduated cylinder
Hydrogen peroxide
Procedure:
1. Place all 5 glass tubes face up in the drying rack
2. Use tweezers and soak one potato cube into water and leave it there for 2 minutes.
3. After 2 minutes, use tweezers and place the potato into the first testing tube.
4. Repeat steps 2 and 3 four more times except soaking the other 4 potatoes in baking soda solution, bleach, vinegar, and lemon juice. Place the potatoes in each tube respectively.
5. Pour hydrogen peroxide into the 50 ml glass beaker. (about 25 ml)
6. Use the pipet and measure up 5 ml of hydrogen peroxide in the graduated cylinder
7. Pour the 5 ml of hydrogen peroxide into the first test tube. Start the timer immediately. Time for 7 minutes. Observe and record.
8. Repeat steps 6 and 7 with the other 4 potatoes.
9. Repeat steps 2-8 twice.
Data
Test Tube
Height of foam after 7mm
Color of the Solution
Observations
1(Water)
1mm
Clear
Fizzes, Bubbles
2(Lemon Juice)
0mm
Clear
Small fizzing, not too violent
3(Vinegar)
0mm
Clear
Nothing happens to it.
4(Baking Soda)
4mm
Neon, yellow, but still clear.
Very active, light foam.
5(Bleach)
5mm
Pale yellow
Foamy, lots of bubbles, white dense foam. (exact same results for both trials)
Results
My results showed bleach having the highest amount of foam 5mm, baking soda with the 2nd highest amount of foam (4mm), water being the third (1mm) and lemon juice and vinegar being last at (0mm). Bleach which was first had the ph of 13, baking soda with the ph of 9, water with the ph of