More specifically, however, this experiment focused on the photo part of photosynthesis, or the light dependent reactions in chloroplasts. Light dependent reactions require the presence of light to function, so that this light can be taken to create ATP and to reduce NADP+ to NADPH. Consequently, light dependent reactions shut down in the absence of light, thereby stopping the production of ATP and NADPH (Sadava et al. 2012). With this information, an experimental hypothesis can be formed that the presence of light will cause the redox activity of the spinach chloroplasts to increase, while the absence of light will cause this activity to decrease. One possible null hypothesis could be that there will be no significant difference in redox activity between spinach chloroplasts that are under light and in the dark. Therefore, the alternative hypothesis must be that there will be a difference in this activity between the two groups of chloroplasts. To test these hypotheses, chloroplasts were extracted from spinach leaves in order to create enriched chloroplasts, which were a vital…
In this study, we are interested in examining the effects of photosynthesis in spinach leaves in the presence of different light conditions. The light conditions that will be tested are no light, white light and green light. Therefore, we hypothesized that the O2 rate of consumption would occur more in the green light condition and CO2 rate of consumption would occur more in the no light condition.…
The purpose of this experiment is to see how different concentrations of carbon dioxide affect the rate of photosynthesis in spinach leaves.…
Figure 2. The rate of photosynthesis in elodea plants with different color light filters which include clear, red, blue, and green. The color that produced the highest rate of photosynthesis was clear at .48, then red at .28, and finally green at…
The objective of this study was to figure out how different light colors affected the rate of photosynthesis in spinach leaves. This was done by taking leaf disks, removing the CO2 and sinking them in beakers. One beaker filled with regular water, the other filled with a mixture of water and sodium bicarbonate. The beakers were then placed in front of the light sources and the amount of disks floating was recorded every minute. In our study the disks exposed to the red light started to float sooner than the…
The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll, the green pigment in leaves, from spinach. In the second lab period you will investigate the interaction of light with chlorophyll. Read in Appendix B the reference section titled "Electronic Structure of Matter" copied from reference 1 before performing experiment investigating the photochemistry…
This experiment is concerned with identifying photosynthetic pigments found in spinach plants and determining the spectrum of light each absorbs. By using paper chromatography, different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources, as scientific advances have led the way to artificial photosynthesis on the path to replace fossil fuels (Nath, 237).…
In this lab, four different types of leaves were tested to see the rate at which each leaf photosynthesized. This lab demonstrates how plants store light, capture light, and use light as energy for reproduction and growth, by photosynthesis. The control in this experiment was spinach, which was tested before any of the other plants were. The other plants that were tested were English Ivy, C4 Plant, and a multicolored plant. Each type of leaf was tested in a sodium bicarbonate solution and a solution of distilled water. It will be shown in the discussion whether the hypothesis made was correct or incorrect. It will be shown in the discussion what could have occurred during the experiment that could have affected the results.…
In this experiment spinach, DPIP, phosphate solutions were placed under various filters and the percent orange light transmittance through the solution was measured to determine the rate of photosynthesis. This experiment was set up to discover how different wavelengths of light affect the rate of photosynthesis in a spinach, DPIP, phosphate solution.…
Based off the results from this lab the student was able to determine and have an overall better understanding of the variables that affect the rate of photosynthesis in a plant. The first variable the student tested in the lab was 30cm of light with CO2 present. The student started this trial with all 10-leaf disks and timed how many leaf disks came up every minute for 15 minutes. One minute into the trial and the student recorded that no leaf disks had yet to rise to the top of the solution. At three minutes into the trial the student recorded that no leaf disks had still yet to rise to the top of the solution.…
Thus, in this experiment, we are required to determine the rate of photosynthesis of leaf disc. But first, we must know that, the rate of photosynthesis and the amount of oxygen produced by a plant is affected by different factors such as light intensity, temperature, amount of water and amount of CO2. Thus, we are investigating effect of light intensity on the rate of photosynthesis in leaf discs by varying the light intensity, and fixing the amount of sodium hydrogen carbonate solution and temperature.…
For this experiment I am investigating how the light intensity as my limiting factor will have in impact on the amount to oxygen produced, to should the rate of photosynthesis. Therefore, all the controlled variables must remain constant, if not there could be an affect on the accuracy of the dependent variable. The CO2 levels are the same for each trial because before placing the sodium hydrogen carbonate i will weigh it with a measuring scale for 1.5 grams. After completing the five measurements for the first trial, I will change the water and add a new amount of sodium hydrogen carbonate to the water, however using the same pondweed stem. If I am able to keep the sodium hydrogen carbonate the same the results should have a similar affect,…
On day one, the red and blue light were our first two colors of light. We did not expect the results we received from trial one, therefore we added a second trial. From our research we concluded that the photosynthetic rate is quickest when blue and violet wavelengths are projected on the discs. However, the spinach discs under the red light had a much higher count after the 10 minutes compared to the blue light. On day 2 when we tested the green and regular light, we also had unexpected results, having three to four more discs rising in the cup with regular light than the other colored light.…
In the results of the photosynthesis experiment it was based on how CO2 would react to the fake and real spinach leaves. The amount of CO2 increases and decreases in the spinach leaves when there is light. For the results there was negative and positive slopes due to the different varies of color lights that was used during the experiment. For the hypothesis for testing effects of light on the spinach leaves was that CO2 decreases when you test light that means that the photosynthesis is also increasing, which means photosynthesis will not take place without light. During the experiment the hypothesis predictions were fake leaves light there was no result with CO2 release.…
I predict that the plant under blue and purple light will have a higher photosynthetic rate because according to the colour spectrum, they are of shorter wavelength thus they have a higher energy. The red light and green light will have lower…