How Does Temperature Affect Lipase
How does temperature affect the rate of reaction for Lipase?
As the temperature increases, so will the rate of enzyme reaction. However, as the temperature exceeds the optimum the rate of reaction will decrease.
I predict that at temperatures above 70°C the enzyme lipase will become denatured and at temperatures below 10°C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately 37°C. I predict that before the optimum temperature the rates will gradually increase and preceding the optimum there will be a drastic decrease in rate until the enzyme is denatured.
I predict that the rate of enzyme activity at 45°C will be half that of 30°C.
I predict that the rate of enzyme activity at 45°C will be half that of 30°C.
Diagram courtesy of: http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm
Diagram courtesy of: http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm
In my controlled assessment I will be investigating the activity of lipase on milk fat at various temperatures so that I can then find an accurate temperature as to when the enzyme works at its optimum; when it becomes inactive and when it denatures. To find when the enzyme denatures is to find out when the bonds of this protein disintegrate and henceforth disable the enzyme from being of any further use. When these bonds break, the protein starts to unfold and loses some its properties. For example, a denatured protein usually becomes less soluble. As an enzyme, it will lose its ability to function as a catalyst. If the stress that is causing the denaturation continues, other changes may occur. Now that the normal structure of the protein is gone, new bonds may be formed, giving it a different shape. The bonds broken in a denatured enzyme is that of which links the polymers to form the amino acids. This means that if lipase were to denature at the higher
As the temperature increases, so will the rate of enzyme reaction. However, as the temperature exceeds the optimum the rate of reaction will decrease.
I predict that at temperatures above 70°C the enzyme lipase will become denatured and at temperatures below 10°C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately 37°C. I predict that before the optimum temperature the rates will gradually increase and preceding the optimum there will be a drastic decrease in rate until the enzyme is denatured.
I predict that the rate of enzyme activity at 45°C will be half that of 30°C.
I predict that the rate of enzyme activity at 45°C will be half that of 30°C.
Diagram courtesy of: http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm
Diagram courtesy of: http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm
In my controlled assessment I will be investigating the activity of lipase on milk fat at various temperatures so that I can then find an accurate temperature as to when the enzyme works at its optimum; when it becomes inactive and when it denatures. To find when the enzyme denatures is to find out when the bonds of this protein disintegrate and henceforth disable the enzyme from being of any further use. When these bonds break, the protein starts to unfold and loses some its properties. For example, a denatured protein usually becomes less soluble. As an enzyme, it will lose its ability to function as a catalyst. If the stress that is causing the denaturation continues, other changes may occur. Now that the normal structure of the protein is gone, new bonds may be formed, giving it a different shape. The bonds broken in a denatured enzyme is that of which links the polymers to form the amino acids. This means that if lipase were to denature at the higher
Bibliography: OCR. (June 2012). GCSE TWENTY FIRST CENTURY SCIENCE ADDITIONAL SCIENCE A A A164 BIOLOGY A A164.