How Does Temperature Affect The Reaction Between Catecholase And Peroxidase

Most of life’s biological processes involve the use of protein catalyst called enzymes, that help speed up chemical reactions within the body without being denatured in the processes. Enzymes can be used continually by temporary binding to a specific substrate in the active site to convert the substrate into a product that the cell needs to perform a specific function. Without the use of catalyst enzymes, homeostasis wouldn’t be able to occur quickly enough which can result in many bodily functions unable to work properly. The focus of this study is to measure the effects of external factors of temperature, pH and enzyme concentration levels and how it effects the catalyst reaction times of catecholase and peroxidase enzymes using potato extract. A review on how enzymes react under certain external conditions gave a good foundation to base this experiments hypothesis when looking at concentration of enzymes, temperature and pH levels.
The independent variable is the concentration of catecholase and peroxidase enzymes in all three solutions.
Hypothesis 2: The second hypothesis when testing for temperature states that if the temperature increases or decreases from room temperature this will result in decreases in enzyme reaction rate. The independent variable is the temperature differential of the three enzyme solutions. The dependent variable is the reaction rate of the enzyme catalyst.
Hypothesis 3: The third hypothesis when testing pH levels states change in pH levels form a neutral pH to an acidic or basic pH level in a solution will case the enzyme to decrease in reaction rate. The independent variable is the change in pH levels, and the dependent variable is the rate of enzyme catalysts.