How To Write A Pglo Lab Report

In this particular situation we didn’t add enough PGLO into the DNA so ours didn’t glow. In the control lab a different outcomes was observed in each of the four plates. In the LB/amp/arabinose agarose plate containing the +pGLO sample, fluorescent green colonies developed. This is because the gene which codes for the fluorescent protein, GFP, is located near the beta lactamase gene on the pGLO plasmid, which protects bacteria from the antibiotic ampicillin. When the cell produced beta lactamase to deactivate ampicillin, the GFP gene was also transcribed, producing the fluorescent protein observed. In the LB/amp plate containing the +pGLO sample white, non florescent cells were observed. While these genes contained the pGLO plasmid and the GFP
This lower number could be a result of sources of error that may be present within the methodology of this lab or potential human error. Our variable test had an even lower transformation efficiency of 0 transformants per microgram. This could be attributed to the lack of transformation solution, but the tests should be repeated to reach a definitive result. Due to the multiple solutions and bacterial plates used in this lab there it is likely that some cross contamination occurred. Though many precautions were taken, such as using disposable pipettes and sterile loops, there is always a chance that these tools could be contaminated before use, or that a new substance, such as bacteria, was introduced from the environment. While this could be improved by using a culture hood or wearing gloves, cross contamination, especially from the environment, can never fully be prevented. In this lab transformation efficiency was used to measure how successfully the plasmid was incorporated into the bacterial cells. While calculating transformation efficiency it was found that it depended highly on the amount of bacteria taken from the starter