Abstract:
The following 2 labs experimented the more enzymes and substrates added to the concentration will effect the reaction rate. Our second lab, we tested enzyme and substrate concentrations to determine the increase of temperature and inhibitor. The enzyme source used in both labs was peroxide, guaiacol is used as a substrate for peroxide. We used Guaiacol, turnip extract, peroxide and distilled water for enzyme and substrate concentration. In the second lab we used the same substances in lab one, however using the temperatures of 4○C (ice bath) , 23○C ( room temperature), 37○C (body temperature) and 60○C (water bath) to determine increase of temperature. The result rates of reaction in enzyme concentration started from a low to high increase, unlike the substrate concentration it started high to low then back up to high reaction. The temperature and inhibitor went from low to high to low in the reaction rate.
Introduction: Enzyme concentration and enzyme …show more content…
Three enzyme concentrations were tested with 0.5, 1.0 and 2.0 mL of turnip extract. Peroxaside, enzyme extracted from turnip, extract turnip was prepared by blending 0.5 g of turnip in 200 mL of water. Following the Low, Medium and High Enzyme Concentration was measured by the control, substrate and enzyme. Threes substrate concentrations were tested with 0.1 ml, 0.2 ml and 0.4 ml of Hydrogen peroxide. Then record absorbance every 20 seconds for 3 minutes. In the second lab we used the enzyme and substrate concentration substances in lab one, however using the temperatures of 4○C (ice bath) , 23○C ( room temperature), 37○C (body temperature) and 60○C (water bath) to determine the increase of temperature. In Inhibitor reaction rate we added hydroxylamine. We tested 0 drop, 1 drop and 5 drops of hydroxylamine inhibitor. Add the inbox to enzyme tube, before mixing and initiating