Ib Bio Enzyme Catalyst Lab
Enzyme Catalysis Lab: Laboratory Write Up
Problem/Question: What happens to the amounts of gas produced in a catalyzed reaction of Hydrogen Peroxide when the catalyst is mixed with an acid?
Theory/Hypothesis: In this experiment the researcher and their team will be performing tests on a catalyst (proteins found in potato) to see what will happen when the catalyst in a Hydrogen Peroxide reaction is treated with an acid before the reaction occurs. The researcher hypothesizes that the after treating the catalyst with an acid the amount of gas pressure produced will decrease.
Variables: The independent variable in this experiment is the pH level/amount of acid added to catalyst. For this experiment the researchers used Hydrochloric Acid (HCL). This variable is independent because it changes throughout the experiment where in one stage the pH level of the catalyst is within a neutral range and the next it is changed to an acidic pH level. The dependent variable in this experiment is the level of gas pressure/amount of gas pressure produced by the reaction, which is relevant to the experiment as it is the value being measured. The controlled variables are the weight/size of each potato slice tested, the testing kit used, and graphing calculator used. Apparatus and Materials: 1. 10 mL Graduated Cylinder 2. 100 mL beaker 3. 1g slices of potato (+/- .1g) 4. 10 mL hydrogen peroxide (+/-.05mL) 5. 5 mL Hydrochloric Acid (+/- .05mL) 6. Gas pressure measure (included in graphing calculator testing kit)
Procedure and Control of Variables:
Stage One: Potato without HCL 1. Place 2g sized piece of cut potato in clean test tube 2. Measure initial gas pressure 3. Place 10 mL of Hydrogen Peroxide in test tube 4. Continue monitoring for 4 minutes, at each 20 second interval record gas pressure 5. After 4 minutes, stop recording and clean materials 6. Repeat this stage of the experiment two more times 7. Clean all
Problem/Question: What happens to the amounts of gas produced in a catalyzed reaction of Hydrogen Peroxide when the catalyst is mixed with an acid?
Theory/Hypothesis: In this experiment the researcher and their team will be performing tests on a catalyst (proteins found in potato) to see what will happen when the catalyst in a Hydrogen Peroxide reaction is treated with an acid before the reaction occurs. The researcher hypothesizes that the after treating the catalyst with an acid the amount of gas pressure produced will decrease.
Variables: The independent variable in this experiment is the pH level/amount of acid added to catalyst. For this experiment the researchers used Hydrochloric Acid (HCL). This variable is independent because it changes throughout the experiment where in one stage the pH level of the catalyst is within a neutral range and the next it is changed to an acidic pH level. The dependent variable in this experiment is the level of gas pressure/amount of gas pressure produced by the reaction, which is relevant to the experiment as it is the value being measured. The controlled variables are the weight/size of each potato slice tested, the testing kit used, and graphing calculator used. Apparatus and Materials: 1. 10 mL Graduated Cylinder 2. 100 mL beaker 3. 1g slices of potato (+/- .1g) 4. 10 mL hydrogen peroxide (+/-.05mL) 5. 5 mL Hydrochloric Acid (+/- .05mL) 6. Gas pressure measure (included in graphing calculator testing kit)
Procedure and Control of Variables:
Stage One: Potato without HCL 1. Place 2g sized piece of cut potato in clean test tube 2. Measure initial gas pressure 3. Place 10 mL of Hydrogen Peroxide in test tube 4. Continue monitoring for 4 minutes, at each 20 second interval record gas pressure 5. After 4 minutes, stop recording and clean materials 6. Repeat this stage of the experiment two more times 7. Clean all